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为分析甜瓜属线粒体基因组的遗传特性,选取甜瓜属11个不同种植物为试验材料,采用成熟花粉DAPI压片染色方法结合树脂半薄切片MTG-DAPI双染色技术,观察花粉贴壁期和成熟期细胞中的线粒体分布。结果显示,所涉及的11种甜瓜属植物均同黄瓜一样,成熟花粉细胞生殖核周围或精细胞中存在线粒体DNA;而作为对照的南瓜和西葫芦的花粉细胞发育到成熟期时生殖核周围没有任何细胞器DNA。上述结果表明,甜瓜属植物线粒体DNA有可能通过花粉进行遗传和散布。这个结果将线粒体基因组父系遗传潜力特性扩展到甜瓜属,为进一步探究植物线粒体基因组的父系遗传机制奠定基础。
In order to analyze the genetic characteristics of the mitochondrial genome of melon, 11 different species of melon were selected as test materials. Mature pollen DAPI tablet staining method and semi-thin resin MTG-DAPI double staining technique were used to observe the pollen adherent and mature stages Mitochondrial distribution in cells. The results showed that all the 11 species of melon were involved in the same cucumber, and the mitochondrial DNA was present in the mature pollen cell nucleus or in the sperm cell. However, the control group did not have any around the reproductive nucleus when the pollen cells of pumpkin and zucchini reached maturity Organelle DNA. The above results indicate that it is possible for mitochondrial DNA of melon to be inherited and dispersed by pollen. This result extends the genetic potential of the mitochondrial genome to the melon genus, laying the foundation for further exploration of the paternal genetic mechanism of plant mitochondrial genome.