论文部分内容阅读
目的探讨中药健脾泻浊方对亚急性镉染毒大鼠肾毒性的影响。方法无特定病原体级SD雄性大鼠37只,随机分为空白对照组(7只)和模型组(30只)。空白对照组予腹腔注射生理氯化钠溶液,模型组大鼠以腹腔注射氯化镉混合液的方法制备亚急性镉中毒模型。于造模成功后,模型组随后被分为氯化镉染毒对照组(10只)、染毒后低剂量治疗组(10只)、高剂量治疗组(10只)共3组。对3组大鼠分别灌胃给予生理氯化钠溶液(10ml.kg-1.d-1)和低剂量(4.23g.kg-1.d-1)、高剂量(8.46g.kg-1.d-1)的中药健脾泻浊方。各组于实验终末抽取血液并留取肝、肾组织测定镉水平,并测定血液中尿素氮(BUN)和肌酐(Cr)水平。结果氯化镉染毒对照组的血、肝、肾镉水平均高于空白对照组(P<0.01),血BUN也高于空白对照组(P<0.05)。采用中药健脾泻浊方治疗后,高剂量治疗组和低剂量治疗组的血镉、肝镉、肾镉与氯化镉染毒对照组比较差异均无统计学意义(P>0.05)。氯化镉染毒对照组、染毒后低剂量治疗组和高剂量治疗组各组组内的肝镉、肾镉分别与血镉比较差异均有统计学意义(P<0.01),肝镉、肾镉均较血镉高;氯化镉染毒对照组的肝镉、肾镉比较差异无统计学意义(P>0.05),但染毒后低剂量治疗组和高剂量治疗组组内的肝镉、肾镉比较差异均有统计学意义(P<0.01),肝镉较肾镉高。结论健脾泻浊方对亚急性镉染毒大鼠没有增加肾的毒性作用,其对体内镉的驱排作用也不明显。
Objective To investigate the effect of Jianpijiangzhuo decoction on nephrotoxicity induced by subacute cadmium in rats. Methods Thirty-seven male SD rats without specific pathogen were randomly divided into blank control group (n = 7) and model group (n = 30). The blank control group was intraperitoneal injection of physiological sodium chloride solution, the model group of rats by intraperitoneal injection of cadmium chloride mixture prepared subacute cadmium poisoning model. After successful modeling, the model group was divided into cadmium chloride exposure control group (10), low dose treatment group (10) and high dose treatment group (10). Three groups of rats were given physiological sodium chloride solution (10ml.kg-1.d-1) and low dose (4.23g.kg-1.d-1), high dose .d-1) Chinese medicine spleen diarrhea side. At the end of the experiment, blood was taken from each group and liver and kidney tissues were collected for determination of cadmium levels. Blood urea nitrogen (BUN) and creatinine (Cr) levels were measured. Results The serum levels of cadmium, cadmium and cadmium in the control group were higher than those in the blank control group (P <0.01), and the levels of BUN in blood were higher than those in the blank control group (P <0.05). After treatment with Chinese medicine Jianpi Xiezhuo, there was no significant difference in the blood cadmium, liver cadmium, kidney cadmium and cadmium chloride exposure between the high-dose treatment group and the low-dose treatment group (P> 0.05). Cadmium chloride, cadmium chloride in the control group, low-dose treatment group and high-dose treatment group were significantly different from those in the control group (P <0.01) Kidney cadmium were higher than blood and cadmium; cadmium chloride cadmium poisoning control group liver cadmium, kidney cadmium difference was not statistically significant (P> 0.05), but the low-dose treatment group and high-dose treatment group liver Cadmium, kidney cadmium differences were statistically significant (P <0.01), liver cadmium than kidney cadmium. Conclusion Jianpixiaozhu decoction does not increase renal toxicity in subacute cadmium-exposed rats, and its effect on the excretion of cadmium in body is not obvious.