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目的 :探讨HPV16、18,VEGF、TGF β1与宫颈鳞癌的发生、发展的关系。方法 :99例宫颈癌患者及 5 4例对照组取患者宫颈分泌物 ,使用荧光基因定量PCR法检测HPV16、18,取宫颈癌组Ⅰ、Ⅱ期HPV16、18阳性患者的鳞癌组织用免疫组化方法进行VEGF、TGF β1检测 ,结果 :宫颈癌组HPV16、18水平明显高于对照组 ,且差异有统计学意义 ,P <0 0 5 ;淋巴结转移组中HPV阳性表达高于HPV阴性表达 ,且差异无统计学意义 ,P >0 0 5 ;VEGF、TGF β1在宫颈鳞癌Ⅰ、Ⅱ期表达呈增高趋势 ,组间比较差异无统计学意义 ,P>0 0 5。结论 :HPV16、18可能为宫颈癌的主要危险因素 ,HPV上调VEGF、TGF β1启动子的活性 ,VEGF可促进肿瘤细胞的增生与转移 ;TGF β1可能与机体免疫功能降低及肿瘤细胞免疫逃逸有关。
Objective: To investigate the relationship between HPV16,18, VEGF, TGFβ1 and the occurrence and development of cervical squamous cell carcinoma. Methods: Totally 99 cervical cancer patients and 54 control subjects were selected for cervical secretions. Fluorescent quantitative PCR was used to detect HPV16,18. The squamous cell carcinoma of HPV16,18 positive patients with cervical cancer Ⅰ, The levels of HPV16,18 in cervical cancer were significantly higher than those in control group (P <0.05). The positive rate of HPV in cervical lymph node metastasis was higher than that in HPV negative, There was no significant difference between the two groups (P> 0.05). The expressions of VEGF and TGFβ1 in stage Ⅰ and stage Ⅱ of cervical squamous cell carcinoma showed a trend of increase. There was no significant difference between the two groups (P> 0.05). Conclusion: HPV16,18 may be the main risk factor for cervical cancer. HPV may up-regulate the activity of VEGF and TGFβ1 promoters. VEGF may promote the proliferation and metastasis of tumor cells. TGFβ1 may be related to the decrease of immune function and tumor cell immune escape.