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目的探讨PTEN和C-erbB-2蛋白在子宫内膜癌中表达的临床意义。方法采用免疫组化S-P法,分别在20例正常子宫内膜组织、20例子宫内膜不典型增生过长,50例术后子宫内膜癌组织标本中检测PTEN和C-erbB-2蛋白的表达情况。结果 (1)子宫内膜癌组织与子宫内膜不典型增生过长、正常子宫内膜组织比较,PTEN蛋白阳性率明显降低,有显著性差异。C-erbB-2蛋白阳性率明显增加,有显著性差异。(2)PTEN蛋白的表达与组织学分级、临床分级及临床分期都有关,而且随着组织学分级和临床分期的升高,PTEN的阳性表达出现显著降低,和有无淋巴结转移无关。(3)C-erbB-2蛋白的表达与临床分期有关,随着临床分期的增加,C-erbB-2蛋白的阳性表达显著升高,与组织学分级及淋巴结转移无显著相关。(4)在子宫内膜癌中PTEN与C-erbB-2蛋白的表达呈负相关。结论 PTEN蛋白失表达和C-erbB-2蛋白过表达在子宫内膜癌的发生、发展中发挥着一定的作用,两者的联合检测可能有助于子宫内膜癌的早期诊断,为预后评估提供帮助。
Objective To investigate the clinical significance of PTEN and C-erbB-2 protein expression in endometrial carcinoma. Methods Immunohistochemical SP method was used to detect the expression of PTEN and C-erbB-2 protein in 20 cases of normal endometrium, 20 cases of endometrial atypical hyperplasia and 50 cases of endometrial carcinoma Express the situation. Results (1) There was a significant difference in the positive rate of PTEN protein between endometrial cancer tissues and atypical hyperplasia of endometrium, compared with normal endometrium tissues. The positive rate of C-erbB-2 protein increased significantly, with significant difference. (2) The expression of PTEN protein was correlated with histological grade, clinical grade and clinical stage. With the increase of histological grade and clinical stage, the positive expression of PTEN was significantly decreased, with or without lymph node metastasis. (3) The expression of C-erbB-2 protein was correlated with clinical stage. With the increase of clinical stage, the positive expression of C-erbB-2 protein was significantly increased, which was not associated with histological grade and lymph node metastasis. (4) The expression of PTEN and C-erbB-2 protein in endometrial carcinoma was negatively correlated. Conclusions PTEN protein loss and C-erbB-2 protein overexpression play an important role in the development of endometrial carcinoma. The combined detection of PTEN protein and protein may be helpful for the early diagnosis of endometrial carcinoma, provide help.