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目的探讨肿瘤坏死因子α-238/-308(TNF-α-238/308)基因位点多态性与乳腺癌患者易感性及临床病理特征的相关性,以此为临床上乳腺癌基因筛查提供参考依据。方法选择中国福建泉州地区134例乳腺癌患者及150例健康体检者为研究对象,采用DNA测序方法检测TNF-α启动子基因G238A、G308A位点的基因多态性,应用统计学方法分析基因型分布于乳腺癌发病风险及临床病理特征的关系。结果 TNF-α-238/308基因型GG、GA、AA的频率在2组中差异无统计学意义(P>0.05)。TNF-α-238/308基因多态性与Cerb B-2、肿瘤分期、肿瘤大小、淋巴结转移及雌孕激素受体状态等参数均无相关性(P>0.05)。结论乳腺癌易感性与TNF-α-238/308基因多态性无相关性,因此不推荐将其单独作为乳腺癌基因筛查的候选指标。
Objective To investigate the relationship between TNF-α-238/308 gene polymorphism and the susceptibility and clinicopathological features of patients with breast cancer. To investigate the relationship between the polymorphisms of tumor necrosis factor α-238 / Provide a reference basis. Methods A total of 134 breast cancer patients and 150 healthy volunteers from Quanzhou, Fujian Province of China were selected as study objects. The gene polymorphisms of TNF-α promoter G238A and G308A were detected by DNA sequencing. The genotypes Distribution in the incidence of breast cancer and clinicopathological characteristics of the relationship. Results There was no significant difference in the frequencies of GG, GA and AA in TNF-α-238/308 genotype between the two groups (P> 0.05). There was no correlation between TNF-α-238/308 gene polymorphisms and Cerb B-2, tumor stage, tumor size, lymph node metastasis and estrogen and progesterone receptor status (P> 0.05). Conclusion There is no correlation between the susceptibility of breast cancer and TNF-α-238/308 gene polymorphism. Therefore, it is not recommended to use it alone as a candidate for breast cancer gene screening.