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为探讨利用TnI-fast基因进行卵巢癌基因治疗的有效性及其机制,将TnI-fast基因cDNA转染人卵巢癌细胞系SKOV3.采用MTT法和流式细胞技术分别检测TnI-fast基因转染、空载体转染和未转染的SKOV3细胞体外生长状态.收集3种细胞培养上清液,检测3种培养上清液对人脐静脉内皮细胞增殖抑制效应.3种细胞分别接种到裸鼠,观察肿瘤生长、细胞凋亡、肿瘤血管生成和TnI-fast基因局部表达.体外试验发现,与空载体转染和未转染的SKOV3细胞比较,TnI-fast基因表达对肿瘤细胞自身的生长无抑制作用,但可抑制人脐静脉内皮细胞增殖.动物实验中,TnI-fast基因表达可显著抑制肿瘤生长,生长抑制率达73%.其肿瘤细胞增殖率与对照组相当,但微血管密度显著降低,细胞凋亡显著增加.提示,肿瘤自身血管生成抑制可显著延缓卵巢癌生长.利用血管生成特异性抑制基因TnI-fast进行抗肿瘤血管生成基因治疗可作为肿瘤治疗的新策略之一.
TnI-fast gene was transfected into human ovarian cancer cell line SKOV3 in order to explore the effectiveness and mechanism of TnI-fast gene therapy for ovarian cancer gene therapy.MTT method and flow cytometry were used to detect TnI-fast gene transfection , Empty vector transfected and untransfected SKOV3 cells in vitro growth conditions collected three cell culture supernatants were detected three kinds of culture supernatant on human umbilical vein endothelial cell proliferation inhibitory effect of three kinds of cells were inoculated into nude mice , And observed tumor growth, apoptosis, tumor angiogenesis and TnI-fast gene expression in vitro.Compared with empty vector transfected and untransfected SKOV3 cells, TnI-fast gene expression on the growth of tumor cells without Inhibition, but can inhibit the proliferation of human umbilical vein endothelial cells.In animal experiments, TnI-fast gene expression can significantly inhibit tumor growth, the growth inhibition rate of 73% .The tumor cell proliferation rate and the control group, but the microvessel density was significantly lower , Significantly increased apoptosis, suggesting that tumor angiogenesis inhibition can significantly delay ovarian cancer growth.Using angiogenesis-specific gene TnI-fast anti-angiogenic gene therapy Can be used as a new strategy for cancer treatment.