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目的探讨磷酸酶及张力蛋白同系物(PTEN)基因5′端非编码区(5′-UTR)-9C→G多态性位点与鼻咽癌发生风险及疾病严重程度的相关性。方法应用PCR-RFLP方法对593例鼻咽癌患者和480例健康对照PTEN基因的-9C→G多态性位点进行分型。采用非条件Logistic回归分析,校正性别、年龄、饮酒状态、吸烟状态、吸烟量的影响,计算比数比(OR)和95%置信区间(CI),评价多态性位点与表型的遗传相关性。结果PTEN基因-9C→G多态性位点与鼻咽癌发生风险无关联,进一步按性别、年龄、饮酒状态、吸烟状态和吸烟量风险因素进行分层分析,仍无相关性。在疾病严重程度分析中,按临床分级及T、M、N分级均未发现-9C→G多态性位点与鼻咽癌疾病严重程度相关。结论PTEN基因-9C→G多态性位点与鼻咽癌的易感性及其严重程度均无关联,该多态性位点可能在鼻咽癌的易感性和疾病严重程度机制中不发挥重要作用。
Objective To investigate the relationship between the 5 ’untranslated region (5’-UTR) -9C → G polymorphism of phosphatase and tensin homolog (PTEN) gene and the risk of developing nasopharyngeal carcinoma (NPC) and the severity of the disease. Methods The polymorphisms of -9C → G polymorphism of PTEN gene in 593 patients with nasopharyngeal carcinoma and 480 healthy controls were genotyped by PCR-RFLP. Non-conditional logistic regression analysis was used to assess the effect of gender, age, drinking status, smoking status and smoking volume, and to calculate the odds ratio (OR) and 95% confidence interval (CI) Correlation. Results There was no correlation between PTEN gene -9C → G polymorphism and the risk of nasopharyngeal carcinoma. Stratified analysis according to sex, age, drinking status, smoking status and smoking risk factors still had no correlation. In the analysis of disease severity, no correlation was found between -9C → G polymorphism sites and the severity of nasopharyngeal carcinoma according to clinical grade and T, M, N grade. Conclusions There is no correlation between PTEN-9C → G polymorphism and the susceptibility and severity of nasopharyngeal carcinoma. The polymorphic loci may not play an important role in the susceptibility and severity of NPC effect.