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目的:研究5F对非小细肺癌NCI-H460细胞survivin、p65和bcl-2 mRNA水平的影响。方法:MTT法检测5F对NCI-H460细胞的生长抑制作用;用半定量RT-PCR方法检测survivin、p65及bcl-2 mRNA水平的变化。结果:5F抑制NCI-H460细胞的生长,其效果与5F的浓度和作用时间相关,24、48、72h的IC50分别为:21.40、4.52、1.02μg·mL-1;100μg.mL-15F作用NCI-H460细胞6h后能引起survivin mRNA水平显著的降低(p<0.05),24h后出现增加(p<0.05);、p65和bcl-2 mRNA水平在作用3h就发生明显减少(p<0.05)。结论:5F诱导NCI-H460细胞凋亡的机制可能是通过抑制survivin、p65和bcl-2mRNA水平来发挥作用的。
Objective: To investigate the effect of 5F on survivin, p65 and bcl-2 mRNA levels in non-small cell lung cancer NCI-H460 cells. Methods: MTT assay was used to detect the inhibitory effect of 5F on NCI-H460 cells. The mRNA expression of survivin, p65 and bcl-2 were detected by semi-quantitative RT-PCR. Results: 5F inhibited the growth of NCI-H460 cells. The effect of 5F was related to the concentration of 5F and the action time. The IC50 values at 24, 48 and 72h were 21.40, 4.52 and 1.02μg · mL-1, respectively. -H460 cells induced a significant decrease of survivin mRNA level (p <0.05), increased after 24h (p <0.05); p65 and bcl-2 mRNA levels decreased significantly after 3h (p <0.05). Conclusion: The mechanism of 5F-induced NCI-H460 cell apoptosis may be through the inhibition of survivin, p65 and bcl-2 mRNA levels.