论文部分内容阅读
目的:探讨miRNA-93在膀胱癌中的表达及对人膀胱癌细胞株T24细胞生物学特性的影响及其作用机制。方法:选取郑州大学附属南阳市中心医院泌尿外科2010年5月至2016年5月收治的79例膀胱癌患者病理组织标本及配对癌旁正常组织,通过qRT-PCR检测miRNA-93的表达水平。沉默miRNA-93后,采用CCK-8法、Transwell实验和流式细胞术分别检测细胞增殖和迁移能力的变化及细胞的凋亡情况;Western blotting检测AKT/p-AKT、GSK3β/p-GSK3β蛋白表达水平的变化。结果:miRNA-93在膀胱癌组织及癌细胞中高表达,且表达水平与癌灶大小、淋巴结转移、病理分级及T分期有关(均P<0.05)。沉默miRNA-93后,T24细胞的增殖能力显著降低(P<0.05),沉默miRNA-93促进T24细胞的凋亡并抑制其迁移;同时,沉默miRNA-93后p-AKT和p-GSK3β的蛋白表达水平均显著下降(P<0.05)。结论:miRNA-93能够促进人膀胱癌细胞株T24细胞的增殖和迁移,并抑制凋亡,其作用机制可能与下调p-AKT、p-GSK3β蛋白表达有关,提示miRNA-93可以作为诊断和靶向治疗膀胱癌的潜在作用位点。
OBJECTIVE: To investigate the effect of miRNA-93 in bladder cancer and its biological characteristics on human bladder cancer cell line T24 and its mechanism. Methods: Totally 79 pathological specimens of bladder cancer and paired para-cancerous tissues were collected from Department of Urology, Nanyang Central Hospital Affiliated to Zhengzhou University from May 2010 to May 2016, and the expression of miRNA-93 was detected by qRT-PCR. After silence of miRNA-93, the cell proliferation and migration ability and cell apoptosis were detected by CCK-8 assay, Transwell assay and flow cytometry respectively. The protein expressions of AKT / p-AKT and GSK3β / p-GSK3β Changes in expression levels. Results: miRNA-93 was overexpressed in bladder cancer tissues and cancer cells. The expression level of miRNA-93 was correlated with tumor size, lymph node metastasis, pathological grade and T stage (all P <0.05). After miRNA-93 silencing, the proliferation of T24 cells was significantly decreased (P <0.05). The silencing of miRNA-93 promoted the apoptosis and inhibited the migration of T24 cells. Meanwhile, the silencing of miRNA-93 p-AKT and p-GSK3β The expression level decreased significantly (P <0.05). Conclusion: miRNA-93 can promote the proliferation and migration of human bladder cancer cell line T24 cells and inhibit its apoptosis. The mechanism may be related to the down-regulation of the expression of p-AKT and p-GSK3β, suggesting that miRNA-93 can be used as a diagnostic and target Potential site of action for the treatment of bladder cancer.