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目的探讨孕酮对人子宫内膜细胞RL95-2骨桥蛋白(OPN)表达的调节作用及其临床意义。方法利用免疫荧光方法观察OPN在RL95-2细胞的表达定位。将不同浓度的孕酮(1×10-9 mol/L、1×10-7 mol/L、1×10-5 mol/L)处理RL95-2细胞24h,采用逆转录聚合酶链反应(RT-PCR)技术和免疫印迹检测(Western blot)技术检测各组OPN mRNA和蛋白的表达水平,以不加孕酮的实验组为空白对照组。结果 OPN定位表达于RL95-2细胞膜表面;与对照组相比,高浓度孕酮(1×10-5 mol/L)组的OPN mRNA和蛋白的表达显著增加(P<0.05),低浓度孕酮(1×10-9 mol/L)组则显著抑制OPN mRNA和蛋白的表达(P<0.05)。结论在一定浓度范围内,孕酮可双向调节子宫内膜表面骨桥蛋白的表达。
Objective To investigate the regulatory effect of progesterone on osteopontin (OPN) expression in human endometrial cell line RL95-2 and its clinical significance. Methods The expression of OPN in RL95-2 cells was observed by immunofluorescence staining. RL95-2 cells were treated with different concentrations of progesterone (1 × 10-9 mol / L, 1 × 10-7 mol / L, 1 × 10-5 mol / L) for 24 h. Reverse transcriptase-polymerase chain reaction -PCR) technique and Western blotting were used to detect the expression of OPN mRNA and protein in each group. The experimental group without progesterone was used as blank control group. Results OPN localization was detected on the surface of RL95-2 cells. Compared with the control group, the expression of OPN mRNA and protein was significantly increased in high concentration progesterone (1 × 10-5 mol / L) group (P <0.05) Ketone (1 × 10-9 mol / L) significantly inhibited OPN mRNA and protein expression (P <0.05). Conclusion Progesterone can regulate the expression of osteopontin on the endometrium in a certain concentration range.