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研究了丁酸钠诱导的HeLa细胞凋亡过程中端粒酶活性和端粒长度的变化. 丁酸钠处理 48 h后, HeLa细胞表现出明显的凋亡特征: 染色质凝集, 形成凋亡小体, DNA在核小体间断裂并降解产生梯状电泳图谱. 凋亡细胞中端粒酶活性无显著改变, 端粒长度却急剧缩短; 抑制端粒酶活性后, 细胞对凋亡刺激的易感性增强, 端粒的降解加快. 以上结果表明, 丁酸钠诱导的细胞凋亡与端粒长度密切相关, 但不影响端粒酶的活性. 端粒酶则可以通过保护端粒来增加细胞对凋亡刺激的耐受性.
The changes of telomerase activity and telomere length during the apoptosis of HeLa cells induced by sodium butyrate were studied. After 48 hours of sodium butyrate treatment, HeLa cells showed obvious apoptotic characteristics: chromatin condensation and small apoptosis. In vivo, DNA breaks down and degrades between nucleosomes to produce a ladder-like electrophoretogram. Telomerase activity in apoptotic cells is not changed significantly, but telomere length is drastically shortened; after inhibition of telomerase activity, cells are susceptible to apoptosis. Perceptual enhancement, accelerated degradation of telomeres. The above results indicate that sodium butyrate-induced apoptosis is closely related to telomere length, but does not affect the activity of telomerase. Telomerase can protect cells by protecting telomeres. Apoptosis stimuli tolerance.