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野生稻资源是水稻育种中获取有利外源基因的一个主要来源,紧穗野生稻(Oryza eichingeri, 2n=24,CC)原产于非洲,具有高抗褐飞虱、白背飞虱和白叶枯病等多种有利性状.在紧穗野生稻与栽培稻(Oryza sativa, 2n= 24, AA)品种 02428远缘杂交后代中,利用限制性片段长度多态性(restriction fragmentlength polymorphism, RFLP)和微卫星(simple sequence repeats, SSR)等分子标记,对栽培稻背景下外源遗传物质的存在进行了跟踪鉴定,并对来自紧穗野生稻的抗褐飞虱基因进行了遗传分析和染色体定位.结果表明,紧穗野生稻的染色体片段已经易位到栽培稻中;抗褐飞虱性状由一对显性主效基因控制,位于第 2染色体,在两个微卫星标记 RM240和 RM250之间,遗传距离分别为 6.1和 5.5 CM,暂时定名为Bph13(t).该基因的发现和定位将有助于对水稻褐飞虱抗性的改良.
Oryza sativa (Oryza eichingeri, 2n = 24, CC) is a native African origin with high resistance to brown planthopper, white-backed planthopper and bacterial leaf blight And many other favorable traits. Using restriction fragment length polymorphism (RFLP) and microsatellite (SSR) markers in distant hybridization progenies of Oryza sativa and Oryza sativa (2n = 24, AA) ) And other molecular markers, the existence of cultivated rice in the background of foreign genetic material were tracked identification, and from the tight-panicle wild rice resistance to BPH gene genetic analysis and chromosome mapping. The results showed that the chromosomal segments of tight-heading wild rice had translocated into cultivated rice. The resistance to brown planthopper trait was controlled by a dominant dominant gene, located on chromosome 2. The genetic distance between two microsatellite markers, RM240 and RM250, 6.1 and 5.5 CM respectively, tentatively named Bph13 (t). The discovery and localization of this gene will help to improve the resistance of rice plants to BPH.