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目的:检验荧光原位杂交法在双歧杆菌属鉴定方面的用途。方法:采用对数生长期的8株双歧杆菌和10株其他厌氧、需氧杆菌在相同的条件下分别与双歧杆菌属特异性16SrRNA寡核苷酸基因探针和细菌界通用16SrRNA寡核苷酸基因探针在载玻片上进行原位杂交,在荧光显微镜下观察杂交结果,拍摄同一视野的荧光显微镜照片和相差显微镜照片,计算杂交率。结果:所用的双歧杆菌菌株均与两种基因探针杂交,在荧光显微镜下杂交菌与黑暗背景对照显著,杂交率在80%以上而其他菌株仅能与细菌界通用探针杂交。结论:所选用的以16SrRNA为靶基因的寡核苷酸探针可用于原位杂交法对双歧杆菌属的鉴定。
Objective: To examine the use of fluorescence in situ hybridization in the identification of Bifidobacterium. Methods: Eight strains of Bifidobacterium and 10 strains of other anaerobic and aerobic bacilli in logarithmic growth phase were used respectively under the same conditions with 16S rRNA gene probes specific to Bifidobacterium and bacterial 16S rRNA oligo The nucleotide gene probes were hybridized in situ on a glass slide, the hybridization results were observed under a fluorescence microscope, the fluorescence microscopy pictures and the phase contrast microscope photographs of the same field were taken to calculate the hybridization rate. Results: The Bifidobacterium strains used were all hybridized with two kinds of gene probes. The results showed that the hybridization rate was more than 80% under the fluorescence microscope under the dark background, while the other strains could only hybridize with the common bacterial probe. Conclusion: The selected oligonucleotide probes targeting 16SrRNA can be used for the identification of Bifidobacterium by in situ hybridization.