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目的:探讨花生四烯酸(AA)代谢物在湿热型溃疡性结肠炎(UC)大鼠发病的作用以及黄芩汤的调节。方法:采用高脂高糖饲料结合人工气候箱法造成大鼠湿热,使用三硝基苯磺酸灌肠造成湿热型UC模型;将造模大鼠分为模型组(n=12)、黄芩汤治疗组(n=13)及美沙拉嗪组(n=12)。治疗两周后用ELISA法检测大鼠前列腺素E2(PGE2)、血小板活化因子(PAF)、白三烯B4(LTB4)及环氧合酶-2(COX-2)含量;检测甘油三酯(TG)及总胆固醇(CH)含量并计算粪便脂肪球数量从而反映湿热程度;对结肠进行肉眼组织学评分从而评价UC的严重程度;将AA代谢物与以上湿热型UC指标进行相关性分析。结果:与空白组(n=12)比较,造模组血清及结肠PGE2、PAF、LTB4及COX-2含量明显升高(P<0.05);治疗后黄芩汤组的PGE2及LTB4均显著低于美沙拉嗪组(P<0.05),但PAF及COX-2无组间差异。湿热指标方面,治疗后黄芩汤组血脂及粪便脂肪球数量均显著低于美沙拉嗪组(P<0.05);UC方面,黄芩汤及美沙拉嗪组的组织学评分无统计学差异。相关性分析显示组织评分与AA代谢物、血脂及粪便脂肪球均呈显著正相关性(r>0.930,P<0.01)。结论:AA代谢物在湿热型UC大鼠血清及结肠明显升高,且与反映湿热的血脂及粪便脂肪球、与反映UC程度的组织学评分均呈正相关性;黄芩汤对代谢物PGE2、LTB4及对湿热指标的调节作用优于美沙拉嗪。
Objective: To investigate the role of arachidonic acid (AA) metabolites in the pathogenesis of damp-heat ulcerative colitis (UC) rats and the regulation of Huangqin Tang. Methods: The model rats were divided into model group (n = 12), Huangqin Tang treatment group (n = 12) Group (n = 13) and mesalazine group (n = 12). Two weeks after treatment, the levels of prostaglandin E2 (PGE2), platelet activating factor (PAF), leukotriene B4 (LTB4) and cyclooxygenase-2 (COX-2) (TG) and total cholesterol (CH) were calculated and the number of fecal fat globules was calculated to reflect the degree of damp-heat. The histopathological score of colon was used to evaluate the severity of UC. Correlation analysis of AA metabolites with the above indexes was conducted. Results: The levels of PGE2, PAF, LTB4 and COX-2 in serum and colon of model group were significantly higher than those in blank group (n = 12) (P <0.05); PGE2 and LTB4 in Huangqin Decoction group were significantly lower than those in blank group Mesalazine group (P <0.05), but PAF and COX-2 had no difference between groups. In terms of dampness and heat index, the serum lipids and the number of fecal fat globules in the Huangqin Decoction group were significantly lower than those in the Mesalazine group (P <0.05). There was no significant difference in the histological scores between the Huangqin Tang and Mesalazine groups in UC. Correlation analysis showed that there was a significant positive correlation between tissue score and AA metabolites, lipids and fecal fat globules (r> 0.930, P <0.01). CONCLUSION: AA metabolites were significantly increased in the serum and colon of damp-heat UC rats, and positively correlated with the lipids and fecal fat globules reflecting the damp-heat, and the histological scores reflecting the degree of UC. The inhibitory effects of Huangqin decoction on the expression of PGE2, LTB4 And the regulation of dampness indicators better than mesalazine.