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Rice edible quality is related to the amylose content ofendasperm.High amylose content would impair thepalatability.Rice waxy gene is responsible for the synthesisof amylose in endosperm.This study was to transfer waxygene into Longtefu(Oryza sativa ssp.indica)mediated byAgrobacterium.Calli from immature embryos of rice were initiallycultured for 4-5d.They were then infected and trans-formed for 15-20 min by A.tumefacien strain EHA 105harboring Ti plasmid p13w4 and carrying anti-waxy gene,
Rice edible quality is related to the amylose content of endosperm. High amylose content would impair the palatability. Rice waxy gene is responsible for the synthesis of amylose in endosperm. This study was to transfer waxy gene into Longtefu (Oryza sativa ssp. Indica) mediated by Agrobacterium. Calli from immature embryos of rice were initially cultured for 4-5d. They were then infected and trans-formed for 15-20 min by A. tumefacien strain EHA 105 harboring Ti plasmid p13w4 and carrying anti-waxy gene,