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作者采用DNA重组技术,在酵母和猴肾(COS)细胞中大量生产HBsAg,并提纯为具有高度免疫原性的蛋白质微胶粒。在酵母中应用的表达载体是pBR322质粒的衍生物。通过将HBsAg adw基因克隆在酵母甘油醛-3-磷酸脱氢酶(GPD)启动子的下端,并用化学合成的DNA代替克隆基因的
The authors used recombinant DNA technology to produce large quantities of HBsAg in yeast and monkey kidney (COS) cells and purified them into highly immunogenic protein micelles. The expression vector used in yeast is a derivative of the pBR322 plasmid. By cloning the HBsAg adw gene at the lower end of the yeast glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter and replacing the cloned gene with chemically synthesized DNA