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研究利用生物信息学方法对美味牛肝菌全基因组中的SSR位点进行了统计分析,并与其他3种担子菌基因组(灰盖鬼伞、裂褶菌、糙皮侧耳)进行了比较。结果表明,在美味牛肝菌基因组中存在2 732个SSR位点,SSR间的平均距离为17.1kb。73.02%的SSR位点分布在基因组中的非编码区,并以单核苷酸和三核苷酸重复类型为主。分布于5?或3?非编译区的SSR的相对丰度最高,为86个/Mb,而分布于编码序列的SSR相对丰度最低,为31个/Mb。同时,高通量筛选出41个长SSR位点设计引物并通过e-PCR进行了验证。最后通过与其他3种担子菌基因组相比,发现SSR数量与基因组大小无关,SSR类型都以短重复类型(单核苷酸至三核苷酸)为主,比较发现美味牛肝菌基因组中的SSR位点数量相对较多,密度也较高。
The bioinformatics method was used to analyze the SSR loci in the genome of Boletus edulis and compared with those of the other three basidiomycetes genomes (Coprinus comatus, Schizophyllum commune, Pleurotus ostreatus). The results showed that there were 2 732 SSR loci in the B. boletus genome with an average distance of 17.1 kb between SSRs. 73.02% of the SSR loci are located in the non-coding region of the genome and are dominated by single nucleotide and trinucleotide repeat types. The SSRs distributed in 5? Or 3? Non-coding regions had the highest relative abundance of 86 / Mb, while the SSRs distributed in the coding sequences had the lowest relative abundance of 31 / Mb. At the same time, 41 long SSR loci were screened by high-throughput design primers and verified by e-PCR. Finally, compared with the other three kinds of basidiomycete genomes, we found that the number of SSRs was not related to the size of the genome. SSR types were mainly from short repeat type (single nucleotide to trinucleotide) The number of SSR sites is relatively high and the density is also high.