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目的:探讨芪泽汤对慢性肾功能衰竭(CRF)大鼠内质网应激(ERS)相关凋亡分子表达的影响。方法:2.5%腺嘌呤ig24 d建立SD大鼠肾衰模型。分为5组:正常组、模型组、尿毒清组(2.5 g.kg-1.d-1)、芪泽汤高、低剂量组(50.4,12.6g.kg-1.d-1),每组10只。造模同时各治疗组ig给药,干预24 d后,观察体重变化,全自动生化分析仪检测大鼠血尿素氮(BUN)、血肌酐(SCr),应用TUNEL法检测肾组织细胞凋亡,Western blot检测CHOP(C/EBP-homologous protein)和半胱氨酸天冬氨酸蛋白酶12(Caspase-12)肾内表达水平。结果:模型组BUN(17.21±4.63)mmol.L-1,SCr(67.00±25.62)μmol.L-1较对照组BUN(4.19±0.77)mmol.L-1,SCr(15.89±2.62)μmol.L-1明显升高(P<0.01);TUNEL结果显示空白组肾小管上皮细胞有少量凋亡,而模型组细胞凋亡数明显增多(P<0.01);模型组CHOP,Caspase-12表达显著增强(P<0.01)。芪泽汤低剂量和尿毒清能抑制慢性肾功能衰竭大鼠肾小管上皮细胞凋亡和CHOP,Caspase-12表达(与模型组相比较P<0.05)。结论:芪泽汤低剂量能保护腺嘌呤导致的大鼠肾损害,可能与其抑制ERS相关凋亡分子CHOP,Caspase-12高表达有关。
Objective: To investigate the effect of Qizetang on ERS-related apoptosis in chronic renal failure (CRF) rats. METHODS: A rat model of renal failure in SD rats was established by 2.5% adenine ig24 d. The rats were randomly divided into 5 groups: normal group, model group, nimuraqing group (2.5 g.kg-1.d-1), Qize soup high and low dose group (50.4,12.6g.kg-1.d-1) Each group of 10. At the same time, the rats in each treatment group were given ig administration. The changes of body weight were observed 24 d after intervention. The blood urea nitrogen (BUN) and serum creatinine (SCr) were measured by automatic biochemical analyzer. Western blot was used to detect the renal expression of CHOP (C / EBP-homologous protein) and Caspase-12. Results: Compared with the control group, BUN (4.19 ± 0.77) mmol.L-1 and SCr (15.89 ± 2.62) μmol in BUN group (17.21 ± 4.63 mmol.L-1, SCr 67.00 ± 25.62 μmol.L- (P <0.01). The results of TUNEL showed that there was a small amount of apoptosis in the renal tubular epithelial cells in the blank group and the apoptosis of the model group was significantly increased (P <0.01). The expression of CHOP and Caspase-12 in the model group was significantly higher than that in the model group Enhanced (P <0.01). Low dosage of Qizetang and Niaoduqing could inhibit renal tubular epithelial cell apoptosis and expression of CHOP and Caspase-12 in chronic renal failure rats (P <0.05 compared with model group). Conclusion: The low dose of Qize decoction can protect adenine-induced renal damage in rats, which may be related to the inhibition of ERS-related apoptosis molecules CHOP, Caspase-12 high expression.