血清谷胱甘肽S-转移酶在小鼠急性乙醇肝损伤中的诊断作用

来源 :中国卫生检验杂志 | 被引量 : 0次 | 上传用户:aiyang1983
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To study the early diagnosis of serum glutathione S-transferase(GST)in ethanol-induced hepatotoxicity in mice.The result showed that the activities of hepatic glutathione peroxidase,glutathione reductase and superoxide dismutase(SOD)were significantly changed in ethanol(5.7 and 11.4g·kg -1 ig)-treated mice.Serum GST activity and hepatic MDA formation were also remarkably increased.However,there were no serum alanine aminotransferase(ALT) and liver pathological changes.In addition,antioxidants sodium ferulate(100mg·kg -1 ig,qd×10) and allimin(10mg·kg -1 ig,qd×10)treatment could reverse the higher activities of serum GST in ethanol(11.4g·kg -1 )-treated mice.These results suggested that serum GST activity is more sensitive than serum ALT in acute ethanol-induced hepatotoxicity. To study the early diagnosis of serum glutathione S-transferase (GST) in ethanol-induced hepatotoxicity in mice. The result showed the activities of hepatic glutathione peroxidase, glutathione reductase and superoxide dismutase (SOD) were significantly changed in ethanol (5.7 and 11.4 g · kg -1 ig) -treated mice. Serum GST activity and hepatic MDA formation were also remarkably increased. Having had no serum alanine aminotransferase (ALT) and liver pathological changes. In addition, antioxidants sodium ferulate (100 mg · kg - 1 ig, qd × 10) and allimin (10 mg · kg -1 ig, qd × 10) treatment could reverse the higher activities of serum GST in ethanol (11.4 g · kg -1) -treated mice.These results suggested that serum GST activity is more sensitive than serum ALT in acute ethanol-induced hepatotoxicity.
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