论文部分内容阅读
通过EMS诱变野生型高粱BTx623种子获得浅绿叶突变体sll1(Sorghum Light-green Leaf 1)。以野生型BTx623为对照,进行成熟期农艺性状调查和苗期生理生化指标测定。结果表明,突变体sll1茎粗和穗柄长与BTx623相比没有显著差异,而株高、穗长、粒数和千粒重均低于BTx623,存在极显著差异,且抽穗期延迟15~20 d。苗期叶片叶绿素测定结果显示,突变体sll1叶绿素a、叶绿素b和总叶绿素含量均极显著低于BTx623,其中叶绿素b含量只有野生型的1/10,推测该突变体是叶绿素b减少型突变体。突变体sll1蛋白总量显著低于BTx623,但脯氨酸含量以及超氧化物歧化酶、过氧化物酶和过氧化氢酶活性均极显著高于BTx623,说明突变体sll1具有较强的渗透调节能力和清除活性氧的能力以维持自身正常生长。
Sorghum Light-green Leaf 1 was obtained by EMS mutagenesis of wild-type Sorghum BTx623 seeds. The wild-type BTx623 as a control, the maturity of agronomic traits and physiological and biochemical indicators of seedling determination. The results showed that there was no significant difference in sll1 stem diameter and ear length compared with BTx623, while the plant height, spike length, grain number and 1000-grain weight were all significantly lower than those of BTx623, with extremely significant differences, and the heading stage was delayed by 15-20 days. The chlorophyll content of seedling leaves showed that the content of chlorophyll a, chlorophyll b and total chlorophyll of mutant sll1 was significantly lower than that of BTx623, and the content of chlorophyll b was only 1/10 of that of wild type. It was presumed that the mutant was chlorophyll b reduced mutant . The total amount of mutant sll1 protein was significantly lower than that of BTx623, but proline content and superoxide dismutase, peroxidase and catalase activities were significantly higher than BTx623, indicating that mutant sll1 has strong osmotic adjustment Ability and the ability to scavenge reactive oxygen species to maintain their normal growth.