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目的 :建立血浆中白藜芦醇苷的反相高效液相色谱分析方法 ,并对其在苗猪体内的药代动力学进行研究。方法 :采用HypersilODS色谱柱 (10 0mm× 2 1mm ,5 μm) ,甲醇 -水 (2 5∶75 )为流动相 ,流速 1 0mL·min-1,检测波长 2 90nm。结果 :方法平均回收率为 93 3 %～ 98 1% ,RSD <6 0 % ,线性范围 0 39～ 12 5 0mg·L-1(r =0 9947) ,最低检测浓度为 0 2 0mg·L-1。结论 :本试验首次建立了白藜芦醇苷血药浓度的HPLC测定方法 ,操作方便 ,结果准确 ,可用于该药的进一步临床药动学研究
Objective : To establish a RP-HPLC method for the determination of resveratrol in plasma and to study its pharmacokinetics in piglets. Methods : A Hypersil ODS column (10 mm × 21 mm, 5 μm), methanol-water (25:75) was used as the mobile phase, the flow rate was 1.0 mL·min-1, and the detection wavelength was 290 nm. RESULTS: The average recovery of the method was 93 3% to 98 1%, RSD < 60%, linear range 0 39 to 12 5 0 mg·L -1 (r = 9947), and the lowest detection concentration was 0 20 mg·L- 1. Conclusion : This experiment establishes HPLC method for determination of plasma concentration of resveratrol for the first time. It is easy to operate and has accurate results. It can be used for further clinical pharmacokinetic study of the drug.