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通过试管法和玻片法检测抗B抗体的效价和亲和力研究了影响抗B杂交瘤细胞抗体分泌的部分因素 ,结果显示 :0 2 5mmol/L ,0 5mmol/L丁酸钠和部分浓度rhIL 6能明显提高抗B抗体的效价和亲和力 ;胰岛素和维甲酸不能提高抗体的效价和亲和力。HAT培养基重筛选可使部分小体积细胞迅速死亡 ,抗B抗体的效价和亲和力明显提高 ,提示丁酸钠 ,rhIL 6和HAT重筛选可提高抗B杂交瘤细胞抗体的分泌
The potency and affinity of anti-B antibodies were determined by tube test and slide method. Some factors affecting the secretion of anti-B hybridoma cells were studied. The results showed that: 0 25 mmol/L, 0 5 mmol/L sodium butyrate and partial rhIL 6 can significantly improve the anti-B antibody titer and affinity; insulin and retinoids can not increase the antibody titer and affinity. Re-screening of HAT medium resulted in the rapid death of some small-volume cells. The potency and affinity of anti-B antibodies were significantly increased, suggesting that rescreening of sodium butyrate, rhIL 6 and HAT can increase the secretion of anti-B hybridoma cell antibodies.