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目的探讨速冻设备与速冻时间对制备冷沉淀质量的影响。方法采用MBF21速冻机与三洋-30℃低温冰箱对制备后冷沉淀分别在30、50 min时间段进行速冻;用全自动凝血分析仪测定不同设备、不同时间冰冻的冷沉淀中凝血因子FⅧ活性和纤维蛋白原含量,判断是否符合冷沉淀质量要求。结果 2种设备30 min内速冻的冷沉淀,纤维蛋白原含量测定均符合要求,合格率100%;FⅧ活性测定速冻机合格率97.5%,低温储血冰箱速冻的合格率62.5%,2者比较有统计学差异(P<0.01)。2种设备50 min速冻的冷沉淀,部分纤维蛋白析出,纤维蛋白原含量测定合格率均在50%以下,差异无统计学意义(P>0.05);FⅧ活性测定符合率明显降低,速冻机符合率为35%、低温储血冰箱符合率为12.5%,2者比较有统计学差异(P<0.05)。结论速冻机能使冷沉淀快速速冻,使冷沉淀质量更有保障;凝血因子FⅧ是不稳定的凝血因子,温度升高活性不断降低,制备时尽量缩短在冰箱外放置的时间,才能保证冷沉淀质量要求。
Objective To investigate the influence of quick freezing equipment and quick freezing time on the quality of cold precipitation. Methods MBF21 quick-freezing machine and Sanyo -30 ℃ low temperature refrigerator were used to make cryoprecipitate after 30 and 50 min, respectively. The coagulation factor FⅧ activity in cryoprecipitate with different equipment and time was measured by automatic coagulation analyzer Fibrinogen content, to determine whether the quality requirements of cryoprecipitate. Results The results showed that the content of fibrinogen in the two devices was within 30 min and the pass rate was 100%. The pass rate of FⅧ activity quick-freezing machine was 97.5% and that of low-temperature blood storing refrigerator was 62.5%. There was a significant difference (P <0.01). 2 kinds of equipments were frozen in 50 min, and some of the fibrin was precipitated. The pass rate of fibrinogen was below 50%, the difference was not statistically significant (P> 0.05). The coincidence rate of FⅧ activity was significantly decreased, The rate was 35%. The coincidence rate of cryogenic blood-refrigerator was 12.5%. There was a significant difference between the two (P <0.05). Conclusion The quick-freezing machine can quick-freeze the cryoprecipitate and ensure the quality of the cryoprecipitate. The coagulation factor F Ⅷ is an unstable clotting factor, and its temperature rising activity is continuously reduced. The time of placing outside the refrigerator should be shortened as much as possible in order to ensure the quality of the cryoprecipitate Claim.