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许多研究表明细胞机械特性和细胞功能的变化密切相关。提出一种基于介电泳(DEP)原理的快速有效的检测细胞生物力学特性变化的方法。NB4细胞(属于白血病细胞株)作为样品细胞,其细胞生理状态的改变借助化疗药物阿霉素(DOX)实现。NB4-DOX细胞由浓度为0.05μmol/L的DOX对NB4细胞药物作用96 h得到。一个具有平行电极的微流控芯片被用来拉伸NB4和NB4-DOX细胞,并对它们的变形能力进行比较。分析对比6V_(p-p)(峰峰值)电压下,3 min时两种细胞的平均弹性力学参数。发现药物处理后的NB4细胞的平均应变量由加药前的0.11变为0.07,杨氏模量由211.8 Pa变为332.9 Pa,剪切弹性模量由83.4 Pa变为121.5 Pa。实验结果表明,NB4细胞在0.05μmol/L的DOX96 h的药物处理下,细胞硬度明显增加。
Numerous studies show that cellular mechanical properties are closely related to changes in cell function. A fast and effective method to detect the changes of biomechanical properties based on dielectrophoresis (DEP) was proposed. NB4 cells (belonging to the leukemia cell line) were used as the sample cells, and the changes in their cellular physiological states were achieved by the chemotherapeutic drug doxorubicin (DOX). NB4-DOX cells were treated with DOX at a concentration of 0.05 μmol / L for 96 h on NB4 cells. A microfluidic chip with parallel electrodes was used to stretch NB4 and NB4-DOX cells and compare their deformability. The mean elastic mechanics parameters of the two kinds of cells at 6 min (peak-to-peak voltage) were analyzed and compared. The average strain of drug-treated NB4 cells was changed from 0.11 before dosing to 0.07, the Young’s modulus was changed from 211.8 Pa to 332.9 Pa, and the shear modulus was changed from 83.4 Pa to 121.5 Pa. Experimental results show that NB4 cells in the 0.05μmol / L DOX96h drug treatment, the cell hardness increased significantly.