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目的研究长链非编码RNA(LncRNA)SPRY4内含子转录本1(SPRY4-IT1)对膀胱癌生长的影响。方法使用qRT-PCR法检测SPRY4-IT1标本和细胞表达水平并分析其临床病理特征之间的关联;使用特异性小干扰RNA(siSPRY4-IT1)转染两种膀胱癌细胞,使用qRT-PCR法检测siSPRY4-IT1效果,再分别以CCK-8实验、MTT实验、划痕实验、ELISA实验和流式细胞术检测SPRY4-IT1对膀胱癌细胞在增殖、迁移和凋亡等方面的影响。结果 SPRY4-IT1在膀胱癌组织中高表达(68.33%,P=0.026 3)。SPRY4-IT1表达水平与病理分级(P<0.05)和TMN分期(P<0.001)有关。SPRY4-IT1在5637(P=0.002 29)和T24(P=0.000 12)细胞中明显高表达。si-SPRY4-IT1可显著降低SPRY4-IT1表达(P<0.001);si-SPRY4-IT1可显著抑制两种细胞的增殖、迁移和增加凋亡(P<0.01)。结论 SPRY4-IT1在膀胱癌标本组织和细胞水平中高表达,有促进膀胱癌细胞生长的作用,在膀胱癌中起着癌基因的作用。
Objective To investigate the effect of long chain non-coding RNA (SPNR) SPRY4 intron 1 (SPRY4-IT1) on the growth of bladder cancer. METHODS: The expression of SPRY4-IT1 was detected by qRT-PCR and the relationship between the expression of SPRY4-IT1 and clinicopathological features was analyzed. Two kinds of bladder cancer cells were transfected with siSPRY4-IT1. The qRT-PCR The effects of SPRY4-IT1 on the proliferation, migration and apoptosis of bladder cancer cells were detected by CCK-8, MTT, scratch, ELISA and flow cytometry respectively. Results SPRY4-IT1 was highly expressed in bladder cancer (68.33%, P = 0.026 3). SPRY4-IT1 expression was correlated with pathological grade (P <0.05) and TMN stage (P <0.001). SPRY4-IT1 was significantly over-expressed in 5637 (P = 0.00229) and T24 (P = 0.00012) cells. The expression of SPRY4-IT1 was significantly reduced by si-SPRY4-IT1 (P <0.001). Si-SPRY4-IT1 significantly inhibited the proliferation, migration and apoptosis of both cells (P <0.01). Conclusion SPRY4-IT1 is overexpressed in bladder cancer tissues and cells, promoting the growth of bladder cancer cells and plays an oncogene role in bladder cancer.