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目的:观察白藜芦醇(resveratrol,RES)对C57BL小鼠急性放射性肺损伤(acuteradiation-induced lung injury,ARILI)的治疗效果,并探讨其机制。方法:将40只C57BL小鼠随机分为0.9%氯化钠溶液组(A组)、RES组(B组)、放射+0.9%氯化钠溶液组(C组)和放射+RES组(D组),每组10只小鼠。采用60Coγ射线单次16Gy大剂量照射小鼠全肺ARILI模型。照射后4h,RES组用RES20mg/kg灌胃,0.9%氯化钠溶液组用等体积0.9%氯化钠溶液灌胃,连续灌胃30d。取小鼠肺组织进行组织病理学观察。采用ELISA法检测小鼠血清转化生长因子β1(transforming growth factor-β1,TGF-β1)、白细胞介素1β(interleukin-1β,IL-1β)和核因子κB(nuclear factor-κB,NF-κB)水平以及血清丙二醛(malondialdehyde,MDA)活性、羟自由基浓度和超氧化物歧化酶(superoxide dismutase,SOD)活力,并检测肺组织中MDA活性。结果:D组小鼠肺组织损伤程度较C组小鼠明显减轻,其肺组织及血清MDA活性、血清羟自由基浓度以及血清TGF-β1、IL1-β和NF-κB表达水平虽然较略高于A组,但与C组相比明显降低,差异有统计学意义(P<0.05);D组小鼠血清SOD活力明显高于C组,但低于A组和B组,差异有统计学意义(P<0.05);B组小鼠各项检测指标与A组相比,差异均无统计学意义(P>0.05)。结论:RES可有效减轻小鼠的ARILI,且对小鼠肺组织几乎无毒性。RES有望成为一种有效而安全的减轻ARILI的药物。
Objective: To observe the therapeutic effect of resveratrol (RES) on acute radiation-induced lung injury (ARILI) in C57BL mice and its mechanism. Methods: Forty C57BL mice were randomly divided into 0.9% sodium chloride solution group (group A), RES group (group B), radiation + 0.9% sodium chloride solution group (group C) and radiation + RES group Group), 10 mice per group. The whole lung ARILI model was irradiated with a single dose of 16Gy with 60Coγ ray. 4h after irradiation, the RES group was orally administered with RES20mg / kg, while the 0.9% sodium chloride solution group was orally administered with an equal volume of 0.9% sodium chloride solution for 30 days. Take the mouse lung tissue for histopathological observation. Serum levels of transforming growth factor-β1 (TGF-β1), interleukin-1β (IL-1β) and nuclear factor-κB (NF- (MDA), hydroxyl radical concentration and superoxide dismutase (SOD) activity in serum were measured. The activity of MDA in lung tissue was measured. Results: The lung injury in group D was significantly lessened than that in group C, and the activity of MDA, the level of serum hydroxyl radical and the levels of serum TGF-β1, IL1-β and NF-κB in lung tissue and serum were slightly higher (P <0.05). The activity of SOD in serum of group D was significantly higher than that of group C, but lower than that of group A and group B, the difference was statistically significant (P <0.05). There was no significant difference in the detection indexes between group B and group A (P> 0.05). Conclusion: RES can effectively reduce the ARILI in mice and is almost non-toxic to mouse lung tissue. RES is expected to become an effective and safe drug to reduce ARILI.