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目的:观察金线莲多糖(ARP)对小鼠脾淋巴细胞体外增殖,细胞周期及分泌IL-2、IFN-γ的影响。方法:MTT法检测ARP单独及协同Con A、LPS对小鼠脾淋巴细胞增殖;流式细胞仪检测细胞周期;ELISA法检测细胞因子IL-2和IFN-γ的含量。结果:在试验浓度范围,ARP对小鼠脾淋巴细胞没有毒性作用,与空白对照组相比,OD值明显升高(P<0.05),在一定范围内呈量-效关系;ARP与Con A或LPS共同作用小鼠脾淋巴细胞时,其OD值明显高于Con A、LPS单独刺激组(P<0.05);与空白对照组相比,ARP组G_0/G_1期淋巴细胞百分率降低(P<0.01),S期、G2/M期淋巴细胞百分率升高(P<0.01);ARP组IL-2、IFN-γ分泌量明显高于空白对照组(P<0.05)。结论:ARP可促进小鼠脾淋巴细胞增殖并与Con A、LPS具有协同作用。其作用机制可能与促使小鼠脾淋巴细胞通过G_1期限制点,加速G_0/G_1期向S期,S期向G2/M期转化进入细胞增殖周期,并且可能与其促进IL-2、IFN-γ的分泌有关。
OBJECTIVE: To observe the effect of polysaccharide (Anemone, indica) on the proliferation, cell cycle, secretion of IL-2 and IFN-γ of splenic lymphocytes in mice. Methods: The proliferation of mouse splenic lymphocytes was detected by MTT assay alone or in combination with Con A and LPS. The cell cycle was detected by flow cytometry. The levels of IL-2 and IFN-γ were measured by ELISA. Results: ARP had no cytotoxic effect on mouse splenic lymphocytes in the range of experimental concentration, OD value was significantly higher than that of blank control group (P <0.05), and there was a dose-effect relationship between ARP and Con A (P <0.05). Compared with the blank control group, the percentage of lymphocytes in G 0 / G 1 phase in ARP group was lower than that in control group (P < 0.01). The percentage of lymphocytes in S phase and G2 / M phase increased (P <0.01). The secretion of IL-2 and IFN-γ in ARP group was significantly higher than that in blank control group (P <0.05). Conclusion: ARP can promote the proliferation of mouse splenic lymphocytes and has synergistic effect with Con A and LPS. The mechanism may be related to the promotion of IL-2, IFN-γ, IL-2 and IFN-γ in mouse splenic lymphocytes by G 1 -targeting point, accelerating G 0 / G 1 phase to S phase and S phase to G2 / M phase. The secretion of.