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常规育种方法培育香型水稻除了育种年限长,且在后代材料选择过程中,香味基因往往容易丢失。而通过回交转育手段将香味性状导入优良的非香型水稻品种中,则要寻找出一种高效的分子检测手段加以辅助鉴定。本研究以稻花香2号、金禾香稻、南韩长粒香、黑香米,以及8个非香稻品种(品系)为试验材料,利用等位基因特异扩增方法对水稻香味基因进行鉴定。结果表明,南韩长粒香、黑香米及非香稻获得长度为585bp和355bp的两条带,而稻花香2号、金禾香稻仅获得长度为577bp的一条带,供试材料的分子检测结果与香味基因型不完全一致,这在一定程度上限制了等位基因特异扩增法在水稻香味遗传改良中的应用。
Conventional breeding method to cultivate fragrant rice is not only long breeding years, and in the process of selection of the offspring materials, the scent genes are often easily lost. However, the introduction of aroma traits into non-fragrant rice varieties through backcrossing means must find out an efficient molecular detection method to assist the identification. In this study, the rice allelopathic genes were identified by using allelopathic specific amplification method with Daohuaxiang 2, Jinhexiang rice, South Korea long grain fragrant rice, black fragrant rice, and 8 non-fragrant rice varieties (lines) as test materials. The results showed that two bands of 585bp and 355bp in length were obtained from long grain, black basmati and non-fragrant rice in South Korea. Only one band with 577bp in length was obtained from Daohuaxiang 2 and Jinhexiang, The result was not completely consistent with the aroma genotype, which limited the application of allele-specific amplification method to genetic improvement of rice scent to a certain extent.