目的:利用高浓度脂肪酸和高浓度胰岛素共同刺激,诱导HepG2细胞形成具有明显脂滴增加特征的脂质堆积肝细胞模型,并评价小檗碱对该细胞模型脂质堆积的改善作用。方法:将HepG2进行细胞培养12h后,分为正常对照组(Con组)、模型对照组(Model组)和阳性药物组(Ber组)。Con组细胞仅用培养液培养, OBJECTIVE: To induce HepG2 cells to form a lipid-accumulated hepatocyte model with obvious lipid droplets increase by co-stimulation with high concentration of fatty acids and high concentration of insulin, and to evaluate the effect of berberine on lipid accumulation in this cell model. Methods: HepG2 cells were cultured for 12 hours and divided into normal control group (Con group), model control group (Model group) and positive drug group (Ber group). Con group cells cultured with only culture medium,