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为进一步研究肾综合征出血热（HFRS）病毒核蛋白（NP）、胰蛋白（MP）及病毒核酸在HFRS患者外周血单个核细胞（PBMC）中的动态变化，揭示HFRSV在患者PBMC中感染、增殖及消亡之规则，应用免疫细胞化学方法测定18例HFRS患者不同病目的95份外周血单个核细胞中的NP和MP抗原。结果表明：第3病日，即可在患者PBMC中检测到Np师和Mp；第3～5病日，病毒结构蛋白表达较强，6～10日明显变弱，第18病目逐渐消失。逆转录-聚合酶键反应（RT－PCR）检测HRFSV－RAN的研究发现，第3病日至14病日均能检测到病毒的存在。RT－PCR技术对临床的早期诊断和流行病学调查提供了一个快速敏感的诊断方法。
To further investigate the dynamic changes of nucleoprotein (NP), tryptophan (MP) and viral nucleic acid in peripheral blood mononuclear cells (PBMCs) of patients with hemorrhagic fever with renal syndrome (HFRS) and to reveal the association between HFRSV infection and PBMC infection, Proliferation and extinction rules, the application of immunocytochemistry in 18 HFRS patients with different pathogenic 95 peripheral blood mononuclear cells NP and MP antigens. The results showed that on the third day, Np and Mp could be detected in the PBMCs of patients. On the third to fifth day, the structural proteins of the virus were strongly expressed, and became obviously weaker on the 6th to 10th days. RT-PCR detection HRFSV-RAN study found that the third day to 14 days can detect the presence of the virus. RT-PCR technology provides a quick and sensitive diagnostic method for clinical early diagnosis and epidemiological investigation.