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目的:观察参苓白术散干预对肺脾两虚型慢性阻塞性肺疾病(COPD)模型大鼠生长激素释放肽(Ghrelin),肥胖抑制素(Obestatin)蛋白表达的影响,基于Ghrelin/Obestatin信号调节通路从分子水平探讨培土生金法治疗COPD的作用机制和科学内涵。方法:60只SPF级Wistar大鼠随机分为空白组、肺脾两虚模型组、参苓白术散组(根据药物干预时间不同,分为28,35,42 d组),每组12只。采用病证结合造模法复制肺脾两虚型COPD大鼠,空白组大鼠吸入空气+气管内注射等量生理盐水+灌服等量生理盐水;模型组及参苓白术散组采用烟熏+气管内滴注脂多糖+灌服冰冷番泻叶浸液建立肺脾两虚型COPD大鼠模型;参苓白术散组给予参苓白术散灌胃,分别于药物干预第28,35,42天处死大鼠,利用免疫组化法及蛋白免疫印迹法测定大鼠下丘脑组织及胃组织中Ghrelin,Obestatin蛋白表达变化。结果:与空白组比较,肺脾两虚模型组大鼠下丘脑及胃组织中Ghrelin,Obestatin蛋白表达及积分吸光度均显著降低(P<0.01);与肺脾两虚模型组比较,参苓白术散组(28,35,42天)大鼠胃、下丘脑组织Ghrelin,Obestatin蛋白表达及积分吸光度均有不同程度升高(P<0.05)。结论:参苓白术散可调节肺脾两虚型COPD模型大鼠能量代谢,改善其营养不良状况,其机制可能通过调节Ghrelin,Obestatin动态平衡从而维持机体能量稳态来实现。
OBJECTIVE: To observe the effect of Shenqi Baizhu Powder on the expression of growth hormone releasing peptide (Ghrelin) and obestatin protein in rats with chronic obstructive pulmonary disease (COPD) due to deficiency of both lung and spleen, and to adjust the expression of Ghrelin/Obestatin signaling The pathway explores the mechanism and scientific connotation of the method of cultivating earthen gold to treat COPD from the molecular level. Methods: 60 SPF Wistar rats were randomly divided into blank group, lung and spleen deficiency model group, and Shenqi Baizhu San group (divided into 28, 35, and 42 d groups according to different drug intervention time). Each group contained 12 rats. The lungs and spleen deficiency type COPD rats were duplicated by the combination of disease and syndrome, and the rats in the blank group were inhaled air + intratracheal injection of the same volume of normal saline and fed the same amount of normal saline; the model group and Shenqibaishusan group were smoked Intratracheal instillation of lipopolysaccharide and irrigate ice-cold senna extract to establish a lung and spleen deficiency COPD rat model; Shenqi Baizhu scattered group to administer Shenqibaishu Powder to the stomach, respectively, in the drug intervention 28, 35, 42 Rats were sacrificed at day, and the expression of Ghrelin and Obestatin protein in rat hypothalamus and stomach tissue were detected by immunohistochemistry and Western blotting. Results:Compared with the blank group, the expression of Ghrelin and Obestatin in the hypothalamus and stomach tissue in the model group of lung and spleen deficiency were significantly decreased (P<0.01). Compared with the model group of lung and spleen deficiency, Shenqi and Atractylodes macrocephala were compared. The expressions of Ghrelin, Obestatin, and absorbance of the whole stomach and hypothalamus were increased in different groups (28, 35, and 42 days) (P<0.05). Conclusion: Shenqibaizhu Powder can regulate the energy metabolism of COPD rats with deficiency of lung and spleen and improve their malnutrition. The mechanism may be achieved by regulating the dynamic balance of Ghrelin and Obestatin to maintain the energy homeostasis of the body.