论文部分内容阅读
为了研究植物源物质对斜纹夜蛾Spodoptera litura离体培养细胞系SL-1的凋亡诱导作用,采用倒置相差显微镜观察了印楝素、喜树碱等9种物质各自对SL-1凋亡小体的浓度效应及时序性。结果表明:印楝素0.1~5.0μg/mL和喜树碱0.5~20.0μmol/L处理SL-1,24~48h后均产生大量典型的凋亡小体;茶皂素、蓖麻碱、黄樟油、丹皮酚、烟碱、苦参碱和博落回碱0.1~20.0μg/mL处理SL-1后,整个观察期72h内均无明显凋亡小体出现,凋亡诱导作用不明显。印楝素0.75μg/mL诱导SL-1细胞凋亡,从凋亡小体判断,处理后0~36h属细胞凋亡早期,36~60h属细胞凋亡中期,60h后为细胞凋亡晚期。喜树碱5.0μmol/L诱导SL-1细胞凋亡,处理后0~24h属细胞凋亡前期,24~54h属细胞凋亡中期,54h后进入细胞凋亡晚期。初步认为印楝素和喜树碱对SL-1有凋亡诱导作用,并具有一定的浓度依赖性和时序性。
In order to study the apoptosis-inducing effect of plant-derived material on the SL-1 cell of Spodoptera litura, the apoptosis of SL-1 was observed by inverted phase-contrast microscope. Nine kinds of substances, azadirachtin and camptothecin, Body concentration effect and timing. The results showed that a large number of typical apoptotic bodies were produced after azadirachtin 0.1 ~ 5.0μg / mL and camptothecin 0.5 ~ 20.0μmol / L for SL-1,24-48h. Tea saponin, ricinine, After treatment of SL-1 with 0.1-20.0 μg / mL of camphor oil, paeonol, paeonol, nicotine, matrine and bogelin, the apoptotic bodies were not observed within 72 hours. Azadirachtin 0.75μg / mL induced apoptosis of SL-1 cells. Judged from the apoptotic bodies, 0 to 36 hours after treatment were the early stages of apoptosis, 36-60 hours of apoptosis in the middle stage, and 60th after the apoptosis. Camptothecin 5.0μmol / L induced the apoptosis of SL-1 cells, 0-24 hours after treatment, belong to the early stage of apoptosis, 24-54h belong to the middle stage of apoptosis, 54h later into the late stage of apoptosis. It is preliminarily believed that azadirachtin and camptothecin induced the apoptosis of SL-1, and had a concentration-dependent and time-dependent manner.