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采用高效液相色谱法测定各类食品样品中的丙二醛含量,建立了对猪肉、方便面、食用油、大白菜、婴儿配方奶粉和饼干等不同食品基质均适用的分析方法,采用样品加标的方法验证了方法的可行性。样品采用酸液恒温振荡提取,硫代巴比妥酸衍生提取液后,采用Agilent Eclipse XDB-C18色谱柱进行分离测定,以0.01 mol/L乙酸铵(A)-甲醇(B)(7+3,V/V)为流动相进行色谱等度分离,流速1.0 mL/min,柱温30℃,检测波长532nm。结果表明,丙二醛含量为0.125~2 mg/kg时线性良好,相关系数不小于0.9997。在0.1,1.0和2.5 mg/kg 3个添加水平下的回收率为61%~98%,日内精密度(相对标准偏差,RSD)小于8.3%,日间精密度小于9.2%,检测限为7.72μg/kg,方法稳定性良好。
The contents of malondialdehyde (MDA) in various food samples were determined by HPLC. The analytical methods suitable for different food matrices such as pork, instant noodles, cooking oil, Chinese cabbage, infant formula and biscuit were established. The samples were spiked The method verifies the feasibility of the method. The samples were extracted by acid bath with constant temperature and the thiobarbituric acid-derived extracts were separated and determined by Agilent Eclipse XDB-C18 column with 0.01 mol / L ammonium acetate (A) -methanol (B) (7 + 3 , V / V) as the mobile phase. The flow rate was 1.0 mL / min, the column temperature was 30 ℃ and the detection wavelength was 532 nm. The results showed that the linearity was good when the content of MDA was 0.125 ~ 2 mg / kg, the correlation coefficient was not less than 0.9997. The recoveries were between 61% and 98% at the three levels of 0.1, 1.0 and 2.5 mg / kg. The intra-day precision (RSD) was less than 8.3%, the intraday precision was less than 9.2% and the detection limit was 7.72 μg / kg, the method is stable.