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背景:肺的功能和结构改变致肺动脉高压是导致肺心病的先决条件,中药肺心合剂能够有效降低肺动脉高压,但其机制尚未完全阐明。目的:比较不同剂量中药肺心合剂与硝苯地平对肺动脉高压大鼠肺血管重建的逆转效应。设计:以实验动物为研究对象的随机对照实验研究。单位:一所医科大学附属医院护理系、一所医学院附属医院、一所省级医院中医科。方法:利用野百合碱(50mg/kg)复制大鼠肺心病模型。雄性Wister大鼠60只,随机分为6组:正常对照组、模型组、肺心合剂低、中、高剂量组及硝苯地平组,每组10只。分别于制模后14d灌胃相应药物,连续用药8d。处死动物后,利用特殊染色结合病理图象分析方法,测定肺小动脉病理及其形态计量学改变。主要观察指标:①光镜观察及肺小动脉图象分析。②各组肺小动脉管壁中膜厚度,管壁中膜厚度与血管外径比值、管壁面积与血管总面积比值、管腔面积与血管总面积比值。结果:肺心合剂及硝苯地平均能明显减轻模型大鼠的肺血管重构(P<0.01),以肺心合剂高剂量组效果最好。但治疗组各指标仍未完全恢复到正常对照组水平。结论:肺心合剂能部分逆转肺血管结构重建,有效降低肺动脉高压。
BACKGROUND: Pulmonary hypertension caused by pulmonary function and structural changes is a prerequisite for pulmonary heart disease. Traditional Chinese Medicine Feixin Mixture can effectively reduce pulmonary hypertension, but its mechanism has not yet been fully elucidated. OBJECTIVE: To compare the reversal effects of different doses of Chinese medicine Feixin Mixture and nifedipine on pulmonary artery remodeling in rats with pulmonary hypertension. Design: A randomized, controlled experimental study of experimental animals. Units: Department of Nursing, Affiliated Hospital of a Medical University, Affiliated Hospital of Medical School, Department of Traditional Chinese Medicine, Provincial Hospital. METHODS: Rat pulmonary heart disease model was replicated using monocrotaline (50 mg/kg). Sixty male Wister rats were randomly divided into 6 groups: normal control group, model group, lung heart mixture low-, medium-, high-dose group, and nifedipine group, 10 in each group. Respectively, the corresponding drugs were intragastrically administered on the 14th day after the model preparation, and the drug was continuously administered for 8 days. After the animals were sacrificed, pathological and histomorphometric changes in the pulmonary arterioles were determined using special staining combined with pathological image analysis. MAIN OUTCOME MEASURES: 1 Light microscope observation and pulmonary arterial image analysis. 2 The ratio of the medial thickness of the pulmonary arterial wall, the ratio of the medial thickness of the vessel wall to the diameter of the vessel, the ratio of the vessel wall area to the total area of the vessel, the ratio of the lumen area to the total area of the vessel. Results: The concentration of Feixin Mixture and Nifedipine could significantly reduce the pulmonary vascular remodeling in model rats (P<0.01). The effect of Feixin Mixture in high dose group was the best. However, the indicators in the treatment group did not fully recover to the normal control group. Conclusion: Feixin Mixture can partially reverse pulmonary vascular structural reconstruction and effectively reduce pulmonary hypertension.