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目的探讨复方薤白胶囊(FFXBC)对野百合碱(Monocrotaline MCT)诱导的肺动脉高压(PH)大鼠肺小动脉、右心室、肺组织病理的影响。方法SD(Sprague-Dawley)大鼠84只,随机分为7组:正常组10只、模型组14只、桂龙咳喘宁组、地塞米松组、卡托普利组、FFXBC高剂量组、FFXBC低剂量组各12只。MCT进行1次性腹腔注射(按60 mg/kg),正常对照组一次性腹腔注射等量的生理盐水。于造模d 2开始给药。给药d 21,每组随机取6只进行平均肺动脉压(mPAP)、平均右心室压(mRVP)测定,每组随机取8只大鼠进行观察肺小动脉、右心室及肺组织病理形态学变化。结果模型组与正常对照组相比,mPAP、mRVP明显增高,差别有明显统计学意义(P<0.01);模型组小动脉及右心室厚度明显增厚,肺小动脉管腔狭窄甚至闭塞,肺组织炎性细胞浸润非常明显。桂龙咳喘宁组、地塞米松组、卡托普利组、FFXBC高剂量组、FFXBC低剂量组与模型组相比,mPAP、mRVP明显下降,差别有统计学意义(P<0.05或P<0.01),肺小动脉及右心室壁增厚减轻;肺组织炎性细胞浸润减轻,组间比FFXBC高剂量组效果明显优于FFXBC低剂量组。结论FFXBC能有效的降低MCT诱导大鼠PH的作用,可能的机制是抑制、改善肺小动脉及右心室重构,减轻MCT引起的炎性细胞浸润现象,有效降低肺动脉压及右心室压。提示FFXBC将是防治PH的很有前途的中药,值得进一步的研究。
Objective To investigate the effect of Fufang Qibai Capsule (FFXBC) on the pathology of pulmonary arterioles, right ventricle and lung tissue in rats with pulmonary hypertension (PH) induced by Monocrotaline MCT. Methods Eighty-four SD rats (Sprague-Dawley) were randomly divided into 7 groups: 10 in the normal group, 14 in the model group, Guilong Kechuanning group, dexamethasone group, captopril group, and FFXBC high dose group. Twelve of the FFXBC low dose groups. One intraperitoneal injection (at 60 mg/kg) was performed by MCT. The normal control group was given an equal volume of saline by intraperitoneal injection. Administration was started at model d2. After dosing d 21, 6 rats in each group were randomly selected for mean pulmonary artery pressure (mPAP) and mean right ventricular pressure (mRVP) measurements. Eight rats in each group were randomly selected for observation of pulmonary arterioles, right ventricle, and lung histopathology. Variety. Results The mPAP and mRVP were significantly higher in the model group than in the normal control group (P<0.01). The thickness of the arterioles and the right ventricle in the model group was significantly thicker, and the pulmonary artery lumen was narrowed or even occluded. Tissue inflammatory cell infiltration is very obvious. Compared with the model group, mPAP and mRVP were significantly decreased in Guilong Kechuanning group, dexamethasone group, captopril group, FFXBC high dose group, and FFXBC low dose group, and the difference was statistically significant (P<0.05 or P). <0.01), Pulmonary arterioles and right ventricular wall thickening reduced; lung tissue inflammatory cell infiltration reduced, the effect of high-dose group than the FFXBC high-dose group was significantly better than FFXBC low-dose group. Conclusions FFXBC can effectively reduce the effect of MCT on PH in rats. The possible mechanism is to inhibit and improve the remodeling of pulmonary arterioles and right ventricle, reduce the inflammatory cell infiltration caused by MCT, and effectively reduce pulmonary artery pressure and right ventricular pressure. It is suggested that FFXBC will be a promising Chinese medicine for the prevention and treatment of PH, and it is worth further study.