论文部分内容阅读
[Objective]This study aimed to establish an optimized suspension culture for hairy roots of Aconitum coreanum( Levl.) Raipaics.[Method] Hairy root of Aconitum coreanum( Levl.) Raipaics A0489 was selected as experimental material,to screen the optimal medium for suspension culture.[Result]Aconitum coreanum( Levl.) Raipaics hairy roots showed different growth conditions in various liquid medium; salt concentration,nitrogen content,sugar content,rotation speed and room temperature had great influences on the growth of Aconitum coreanum( Levl.) Raipaics hairy roots.[Conclusion]The optimal suspension culture system for Aconitum coreanum( Levl.) Raipaics hairy roots was established: B5was used as basic medium,culture temperature was 20 ℃ under dark conditions,rotation speed was 140 r/m in the first 15 d and 110 r/m in the latter 15 d.
[Method] This study aimed to establish an optimized suspension culture for hairy roots of Aconitum coreanum (Levl.) Raipaics. [Method] Hairy root of Aconitum coreanum (Levl.) Raipaics A0489 was selected as experimental material, to screen the optimal medium for Aconitum coreanum (Levl.) Raipaics hairy roots showed different growth conditions in various liquid medium; salt concentration, nitrogen content, sugar content, rotation speed and room temperature had great influences on the growth of Aconitum coreanum (Levl. Raipaics hairy roots. [Conclusion] The optimal suspension culture system for Aconitum coreanum (Levl.) Raipaics hairy roots was established: B5was used as basic medium, culture temperature was 20 ° C under dark conditions, rotation speed was 140 r / m in the first 15 d and 110 r / m in the latter 15 d.