论文部分内容阅读
自从采用杂交细胞制备特异性MCA成功以来,MCA被迅速应用于许多免疫化学和生物化学技术中。然而,这些高度均质的试剂在琼脂凝胶中往往不产生晶格形成所必需的多价抗原决定簇的交连。本文介绍用~(125)I标记的MCA和一种比较地非特异性兔抗血清相结合后用于交叉免疫电泳和放射电泳免疫上,以检测哺乳动物硫代谢中一种重要的γ-胱硫醚酶(以下简称γ-酶),这类技术已广泛
MCA has been rapidly used in many immunochemical and biochemical technologies since the success of using hybrid cells to produce specific MCA. However, these highly homogeneous reagents often do not produce cross-linking of multivalent antigenic determinants necessary for lattice formation in agar gels. This article describes the use of ~ (125) I-labeled MCA in combination with a relatively non-specific rabbit antiserum for cross immunophoresis and radioimmunoassay to detect an important gamma-cystathionine in mammalian sulfur metabolism Ether enzyme (hereinafter referred to as γ-enzyme), such technologies have been widely