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背景与目的:鼻咽癌治疗首选放射治疗,其疗效与临床分期的早晚明显相关。因此采用新的技术和方法以进一步提高早期诊断率,进而提高疗效一直是鼻咽癌临床研究的重要课题。本研究通过分析碳酸酐酶Ⅸ(CAⅨ)蛋白和无嘌呤/无嘧啶内切核酸酶(apurinic/apyrimidinic endonuclase,APE)表达与鼻咽癌患者临床特征的关系,探讨其在鼻咽癌诊断和治疗中的价值。方法:应用免疫组织化学法检测54例鼻咽癌和20例鼻咽慢性炎性组织中CAⅨ蛋白和APE蛋白的表达,并对10例鼻咽癌患者放疗前和放疗期间组织中CAⅨ蛋白和APE表达水平进行动态检测。结果:鼻咽癌组织中CAⅨ的阳性表达率显著高于鼻咽慢性炎性组织(P<0.01);CAⅨ阳性表达与患者的性别、年龄、T分期、有无颈淋巴结转移和临床分期均无相关性(P>0.05)。鼻咽癌组织中APE的细胞核阳性表达率高于鼻咽慢性炎性组织,其差异具有统计学意义(P<0.05);APE阳性表达与患者的性别、年龄、T分期、有无颈淋巴结转移和临床分期均无关(P>0.05)。放疗后获得肿瘤局部控制的患者,其放疗期间CAⅨ阳性表达率显著低于放疗前(P<0.05)。鼻咽癌中CAⅨ和APE的阳性表达未见相关性(r=-0.028,P>0.05)。结论:CAⅨ的高表达和APE细胞核内高表达可能在鼻咽癌的发生、发展中起重要作用。
BACKGROUND & OBJECTIVE: Nasopharyngeal carcinoma is the first choice of radiotherapy and the curative effect is obviously correlated with the clinical stage. Therefore, the use of new technologies and methods to further improve the early diagnosis rate, and thus improve the efficacy of NPC has been an important issue in clinical research. In this study, the relationship between the expression of carbonic anhydrase Ⅸ (CAIX) protein and apurinic / apyrimidinic endonuclase (APE) and the clinical features of patients with nasopharyngeal carcinoma was analyzed in the diagnosis and treatment of nasopharyngeal carcinoma In the value. Methods: The expressions of CAⅨ protein and APE protein in 54 cases of nasopharyngeal carcinoma and 20 cases of nasopharyngeal chronic inflammatory tissues were detected by immunohistochemistry. The expressions of CAⅨ protein and APE protein in 10 nasopharyngeal carcinoma patients before and during radiotherapy Expression level for dynamic detection. Results: The positive expression rate of CA Ⅸ in nasopharyngeal carcinoma was significantly higher than that in chronic nasopharyngeal tissues (P <0.01). The positive expression of CA Ⅸ was not correlated with the gender, age, T stage, cervical lymph node metastasis and clinical stage Correlation (P> 0.05). The positive rate of APE nucleus in nasopharyngeal carcinoma was higher than that in nasopharyngeal chronic inflammatory tissues (P <0.05). The positive expression of APE was related to the gender, age, T stage, presence or absence of cervical lymph node metastasis There was no correlation between clinical stage and clinical stage (P> 0.05). In patients undergoing local tumor control after radiotherapy, the positive rate of CAⅨ during radiotherapy was significantly lower than that before radiotherapy (P <0.05). No correlation was found between the positive expression of CAIX and APE in NPC (r = -0.028, P> 0.05). CONCLUSION: High expression of CAⅨ and high expression of APE nucleus may play an important role in the occurrence and development of nasopharyngeal carcinoma.