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目的探讨Tim-3基因启动子区rs10053538、rs10515746位点多态性与原因不明复发性流产的关系。方法收集148例原因不明复发性流产患者为研究对象,以153例正常妊娠者为对照。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法和特异性等位基因聚合酶链反应(AS-PCR)法分别检测rs10053538、rs10515746位点多态性,并取部分PCR产物作测序验证。结果原因不明复发性流产患者rs10053538位点GT+TT基因型及T等位基因分布频率(10.1%和5.1%)与对照组(11.8%和6.5%)比较差异无统计学意义(χ2=0.205,P=0.651;χ2=0.592,P=0.441)。rs10515746位点GT+TT基因型频率及T等位基因分布频率(6.8%和3.4%)与对照组(3.9%和2.0%)比较差异均无统计学意义(χ2=1.201,P=0.273;χ2=1.169,P=0.280)。结论 Tim-3基因启动子rs10053538、rs10515746位点多态性与原因不明复发性流产可能无相关性。
Objective To investigate the relationship between rs10053538 and rs10515746 polymorphisms in Tim-3 gene promoter and unexplained recurrent spontaneous abortion. Methods A total of 148 patients with unexplained recurrent spontaneous abortion were collected and compared with 153 normal pregnant women. The rs10053538 and rs10515746 polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and specific allele polymerase chain reaction (AS-PCR) Products for sequencing verification. Results The frequencies of GT + TT genotypes and T alleles (10.1% and 5.1%) in rs10053538 patients with unexplained recurrent spontaneous abortion were not significantly different from those in controls (11.8% and 6.5%) (χ2 = 0.205, P = 0.651; χ2 = 0.592, P = 0.441). rs10515746 GT + TT genotype frequency and T allele distribution frequency (6.8% and 3.4%) compared with the control group (3.9% and 2.0%) showed no significant difference (χ2 = 1.201, P = 0.273; χ2 = 1.169, P = 0.280). Conclusion There is no correlation between rs10053538 and rs10515746 polymorphisms in Tim-3 gene promoter and unexplained recurrent spontaneous abortion.