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冷调节蛋白(cold regulated proteins,CORPs)是植物在冷驯化下产生的特异性蛋白,与植物的抗寒性密切相关。然而,大量研究表明,绝大多数植物冷诱导基因同样会响应水分胁迫。采用半定量RT-PCR分析天山雪莲(Sasussured in-volucrata)冷调节蛋白基因siCOR的表达,结果表明siCOR是一个受干旱胁迫诱导表达的基因。为研究siCOR基因是否与抗旱性相关,以siCOR转基因烟草为研究材料,利用水分胁迫处理进行抗旱性分析。结果表明与野生型(wild-type,WT)相比,转siCOR植株叶片萎蔫较迟且程度较轻,复水后恢复快且较完全;其叶片相对含水量和PSII相对量子产率的降低幅度、相对电导率和丙二醛含量的升高幅度均低于野生型烟草植株。采用PEG6000模拟干旱胁迫,发现转siCOR植株T3代种子的萌发率较高,主根生长的受抑制程度较野生型轻。以上结果表明,siCOR基因在植物对干旱胁迫的响应中起重要作用。
Cold regulated proteins (CORPs) are specific proteins produced by plants under cold acclimation, which are closely related to the cold resistance of plants. However, a large number of studies show that most plant cold-inducible genes also respond to water stress. Semi-quantitative RT-PCR was used to analyze the expression of siCOR in the cold-regulated protein gene of Sasussured in-volucrata. The results showed that siCOR was a gene induced by drought stress. In order to study whether siCOR gene is related to drought resistance, siCOR transgenic tobacco was used as research material, and drought resistance analysis was conducted by using water stress treatment. The results showed that compared with the wild-type (WT), the leaves of transgenic siCOR plants had a later and lesser degree of wilting, recovered faster and more completely after rehydration, and the relative water content of leaves and the relative quantum yield of PSII decreased , Relative electrical conductivity and MDA content increased less than wild type tobacco plants. Using PEG6000 to simulate drought stress, we found that the seed germination rate of T3 generation of siCOR plants was higher than that of wild-type. The above results show that the siCOR gene plays an important role in the response of plants to drought stress.