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为揭示小麦生理型雄性不育的分子机理,更好地为小麦杂种优势利用提供理论依据和技术支撑,本研究以SQ-1诱导的西农558生理型雄性不育的花药为试验材料,以未经SQ-1处理的西农558的花药为对照,利用基因芯片技术对两者的基因表达差异进行了分析,并对部分基因运用半定量PCR技术进行了验证.结果表明,在55 052个转录本中,两材料间差异表达的转录本有2 052条,其中1 294个基因表达上调,758个基因表达下调.功能分类表明这些基因主要参与了毒性物质响应、逆境响应、多糖代谢及信号转导等重要生命过程.为验证芯片数据的可信性,利用cDNA半定量PCR法对11个差异表达显著的基因(Ta.116,Ta.5629,TaAffx.122333,Ta.30726,Ta.13682,Ta.4057,Ta.4101,Ta.4139,Ta.11957,Ta.25934,Ta.27552)进行验证.结果证明,无论是上调表达的还是下调表达的基因,其表达模式都与基因芯片的检测结果的一致.这些基因可作为育性相关候选基因开展下一步研究.
In order to reveal the molecular mechanism of physiological male sterility in wheat and to provide theoretical basis and technical support for the utilization of heterosis in wheat, we used SQ-1 induced anther of male sterile MSG The anther of XiNong558 without SQ-1 was used as a control, the gene expression differences between the two genes were analyzed by gene chip technology, and some genes were verified by semi-quantitative PCR.The results showed that in 55 052 In the transcript, there were 2 052 transcripts differentially expressed between the two materials, of which 1 294 genes were up-regulated and 758 genes were down-regulated.The functional classification indicated that these genes were mainly involved in the response of toxic substances, stress response, polysaccharide metabolism and signal Transduction and other important life processes.In order to verify the reliability of the chip data, 11 differentially expressed genes (Ta.116, Ta.5629, TaAffx.122333, Ta.30726, Ta.13682 , Ta.4057, Ta.4101, Ta.4139, Ta.11957, Ta.25934, Ta.27552) .The results showed that, both the up-regulated expression and down-regulated genes expression patterns were in line with the gene chip The test results are consistent. These genes can be To carry out further research into fertility-related candidate genes.