论文部分内容阅读
目的探讨肿瘤坏死因子α诱导蛋白8(TNFAIP8)在患者胃癌组织中的表达及其与临床病理特征的相关性,体外研究TNFAIP8对胃癌细胞株SGC7901转移能力的影响。方法应用免疫组织化学染色SABC法检测30例胃癌及癌旁组织中TNFAIP8蛋白表达,分析其与临床病理特征相关性。体外培养人胃癌细胞株SGC7901,脂质体介导重组质粒pcDNA3.0-TIPE转染SGC7901,通过Transwell实验检测转染后胃癌细胞侵袭与迁移能力的差异。结果癌旁组织中TNFAIP8的表达与胃癌组织具有明显差异(P<0.001),TNFAIP8蛋白的表达水平与性别、年龄、肿瘤大小无相关性(P>0.05);TNFAIP8蛋白表达水平与肿瘤浸润深度、淋巴结转移情况相关,差异有统计学意义(P<0.05)。转染pcDNA3.0-TIPE的SGC7901细胞较转染pcDNA3.0对照组细胞其迁移与侵袭能力具有明显差异(P<0.001)。结论 TNFAIP8在胃癌的发生发展、转移中可能发挥重要的作用,TNFAIP8有可能成为新的治疗靶点。
Objective To investigate the expression of tumor necrosis factor-α-induced protein 8 (TNFAIP8) in gastric cancer and its relationship with clinicopathological features. To study the effect of TNFAIP8 on metastasis of gastric cancer cell line SGC7901 in vitro. Methods Immunohistochemical SABC method was used to detect the expression of TNFAIP8 protein in 30 cases of gastric cancer and its adjacent tissues. The correlation between TNFAIP8 and clinicopathological features was analyzed. The human gastric cancer cell line SGC7901 was cultured in vitro, and the liposome-mediated recombinant plasmid pcDNA3.0-TIPE was transfected into SGC7901. The transwell assay was used to detect the difference of invasion and migration of gastric cancer cells. Results The expression of TNFAIP8 in adjacent tissues was significantly different from that in gastric carcinoma (P <0.001). There was no correlation between the expression of TNFAIP8 and sex, age and tumor size (P> 0.05). The expression of TNFAIP8 was correlated with the depth of tumor invasion, Lymph node metastasis, the difference was statistically significant (P <0.05). SGC7901 cells transfected with pcDNA3.0-TIPE cells transfected with pcDNA3.0 control group had a significant difference in migration and invasion (P <0.001). Conclusion TNFAIP8 may play an important role in the development and metastasis of gastric cancer. TNFAIP8 may be a new therapeutic target.