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目的探讨载脂蛋白E(ApoE)基因多态性与云南省纳西族T2DM的相关性。方法选择云南省纳西族T2DM患者(T2DM组)300例和正常对照者(NC组)300名,采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测ApoE基因的多态性。结果(1)T2DM组ApoE基因型E3/4、E2/2、E3/3及E4/4的频率分布分别为14.4%、4.7%、80.9%及0.0%。T2DM组与NC组ApoE基因型分布比较,差异有统计学差异(P=0.001),等位基因频率分布比较,差异亦有统计学意义(P<0.001);(2)E3/3基因亚组SUA高于E2/2基因组(P=0.001);(3)Logistic回归分析表明,ApoE基因多态性与云南纳西族T2DM发生无相关性(P>0.05),WC、LDL-C、SUA是T2DM的危险因素(P<0.05)。结论 ApoE基因多态性在云南省纳西族人群中存在种族差异;SUA与ApoE基因多态性有相关性;ApoE基因多态性与云南省纳西族T2DM发生无相关性。
Objective To investigate the association between apolipoprotein E (ApoE) gene polymorphism and T2DM in Naxi Yunnan Province. Methods 300 cases of T2DM patients (T2DM group) and 300 normal controls (NC group) of Yunnan Naxi patients were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) State. Results (1) The frequency distribution of ApoE genotype E3 / 4, E2 / 2, E3 / 3 and E4 / 4 in T2DM group were 14.4%, 4.7%, 80.9% and 0.0% respectively. There were significant differences in the distribution of ApoE genotype between T2DM group and NC group (P = 0.001), allele frequency distribution was also statistically significant (P <0.001). (2) E3 / 3 gene subgroup (3) Logistic regression analysis showed that ApoE gene polymorphism had no correlation with T2DM in Naxi Yunnan (P> 0.05), WC, LDL-C and SUA were T2DM Risk factors (P <0.05). Conclusions ApoE gene polymorphism has ethnic differences in Naxi people in Yunnan Province. SUA is associated with ApoE gene polymorphism. There is no correlation between ApoE gene polymorphism and T2DM in Naxi Yunnan Province.