论文部分内容阅读
目的:探讨丙戊酸钠(VPA)对体外培养的人喉癌Hep-2细胞凋亡的影响及其机制。方法:不同浓度VPA处理人喉癌Hep-2细胞不同时间后,采用MTT法检测细胞的增殖活性,流式细胞仪检测凋亡率,RT-PCR检测凋亡抑制蛋白Survivin mRNA表达的变化。结果:VPA对人喉癌Hep-2细胞的生长具有明显的增殖抑制作用,其作用表现为剂量依赖性和时间依赖性(P<0.01)。流式细胞仪检测发现:以3 mmol/L的VPA处理Hep-2细胞后,其凋亡率呈时间依赖性上升(P<0.01)。RT-PCR检测发现人喉癌Hep-2细胞Survivin mRNA表达呈时间依赖性下调(P<0.01)。结论:VPA对人喉癌Hep-2细胞具有明显的增殖抑制和诱导凋亡的作用,其作用机制与下调Survivin表达比例有关。
Objective: To investigate the effect and mechanism of VPA on the apoptosis of human laryngeal carcinoma Hep-2 cells cultured in vitro. Methods: The proliferative activity of human laryngeal carcinoma Hep-2 cells treated with different concentrations of VPA was detected by MTT assay. The apoptosis rate was detected by flow cytometry. The expression of Survivin mRNA was detected by RT-PCR. Results: VPA could inhibit the proliferation of human laryngeal carcinoma Hep-2 cells in a dose-dependent and time-dependent manner (P <0.01). Flow cytometry showed that the apoptosis rate of Hep-2 cells treated with 3 mmol / L VPA increased in a time-dependent manner (P <0.01). Survivin mRNA expression in Hep-2 cells was down-regulated in a time-dependent manner by RT-PCR (P <0.01). CONCLUSION: VPA has a significant effect on inhibiting proliferation and inducing apoptosis of human laryngeal carcinoma Hep-2 cells. Its mechanism is related to the down-regulation of Survivin expression.