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目的探讨子痫前期患者体内一氧化氮合酶(nitric oxide synthase,NOS)及其主要内源性抑制物非对称二甲基精氨酸(asymmetric dimethylarginine,ADMA)的表达及意义。方法分别选取重度子痫前期、轻度子痫前期、妊娠期高血压患者各20例,另选同期行剖宫产的单胎初产健康妊娠妇女20例作为对照。用Griess法检测各组胎盘组织总NOS活性,免疫组化SP法检测胎盘组织e NOS和i NOS表达。高效液相色谱法检测各组血浆和脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)内ADMA水平。结果重度、轻度子痫前期组胎盘组织总NOS活性分别为(10.4±3.0)、(14.8±1.6)U/mg蛋白质,显著低于对照组(P<0.05);重度、轻度子痫前期组胎盘组织e NOS表达显著低于对照组(P<0.05),重度子痫前期组i NOS表达显著高于对照组(P<0.05);重度子痫前期、轻度子痫前期及妊娠期高血压3组血浆和HUVECs内ADMA水平较对照组均有显著性升高(P<0.05),且4组血浆与HUVECs内ADMA水平呈正相关关系(r=0.873)。结论 NOS与其抑制物ADMA的相互作用对于调节体内NO水平及其生物学功能的发挥起着重要作用,两者可能在子痫前期的发病中起重要作用。
Objective To investigate the expression and significance of nitric oxide synthase (NOS) and its major endogenous inhibitor asymmetric dimethylarginine (ADMA) in preeclampsia. Methods Twenty patients with severe preeclampsia, mild preeclampsia and gestational hypertension were enrolled in this study. Twenty healthy mothers with singleton primiparas during the same period were selected as controls. The total NOS activity of placenta was detected by Griess method. The expression of eNOS and iNOS in placenta was detected by immunohistochemical SP method. The levels of ADMA in plasma and human umbilical vein endothelial cells (HUVECs) were detected by high performance liquid chromatography (HPLC). Results The total NOS activity in the placenta of severe and mild preeclampsia group was (10.4 ± 3.0) and (14.8 ± 1.6) U / mg protein, respectively, which was significantly lower than that of the control group (P <0.05); severe and mild preeclampsia The expression of eNOS in placenta was significantly lower than that in control group (P <0.05), and the expression of iNOS in severe preeclampsia group was significantly higher than that in control group (P <0.05). The levels of eNOS in severe preeclampsia, mild preeclampsia and gestational hypertension There was a significant increase in ADMA levels in blood plasma and HUVECs (P <0.05), and there was a positive correlation between plasma ADMA levels and HUVECs (r = 0.873). Conclusion The interaction between NOS and its inhibitor ADMA plays an important role in the regulation of NO level and its biological functions, both of which may play an important role in the pathogenesis of preeclampsia.