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目的探讨候选抑癌基因OPCML(opioid-binding protein/cell adhesion molecule-like)在人乳腺癌组织中的蛋白表达及临床意义。方法应用免疫组化方法检测117例人乳腺癌组织、41例癌旁组织及24例乳腺腺病组织中的候选抑癌基因OPCML蛋白表达情况,结合临床病理资料进行统计学分析。结果 117例人乳腺癌组织、41例癌旁组织及24例乳腺腺病组织中OPCML蛋白的阳性表达率分别为19.66%(23/117)、90.24%(37/41)、70.83%(17/24),3种乳腺组织中的表达差异有统计学意义(P<0.05);在117例人乳腺癌组织中OPCML蛋白表达与组织学分级、淋巴结是否转移、pTNM分期显著相关(P<0.05);OPCML蛋白表达与Ki-67(r=-0.261)、HER-2(r=-0.294)、EGFR(r=-0.242)呈负相关关系(P<0.05)。结论候选抑癌基因OPCML在人乳腺癌组织中呈现低表达或表达缺失,且与Ki-67、HER-2及EGFR的表达呈负相关,可能在乳腺癌的发展中起抑癌作用。
Objective To investigate the protein expression of candidate tumor suppressor gene OPCML (opioid-binding protein / cell adhesion molecule-like) in human breast cancer and its clinical significance. Methods Immunohistochemistry was used to detect the expression of OPCML, a candidate tumor suppressor gene in 117 cases of breast cancer, 41 cases of paracancerous tissues and 24 cases of breast adenosis. Statistical analysis was performed with clinicopathological data. Results The positive rates of OPCML protein in 117 cases of breast cancer tissues, 41 cases of paracancerous tissues and 24 cases of breast adenosis tissues were 19.66% (23/117), 90.24% (37/41) and 70.83% (17 / 24). The expression of OPCML protein in 117 cases of human breast cancer was significantly correlated with histological grade, lymph node metastasis and pTNM stage (P <0.05) The protein expression of OPCML was negatively correlated with Ki-67 (r = -0.261), HER-2 (r = -0.294) and EGFR (r = -0.242) (P <0.05). CONCLUSION: The candidate tumor suppressor gene OPCML is low or absent in human breast cancer tissues and negatively correlated with the expression of Ki-67, HER-2 and EGFR, which may play a role in the development of breast cancer.