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目的对2012-2013年广西桂林地区的手足口病病例进行肠道病毒71型(EV71)相关病原学分析。方法收集手足口病病例样本737份,采用RT-PCR方法进行检测,选取EV71阳性样本分离病毒,将获取的毒株经RT-PCR方法扩增VP1基因序列并测序,对VP1基因进行进化分析。结果 737份病例中,荧光PCR检测出EV71感染共169例,阳性率为22.93%,测序获得的89株EV71的VP1基因核苷酸及氨基酸序列同源性为95.01%~100.00%和98.47%~100.00%,与C4亚型代表株核苷酸同源性大于95%,氨基酸同源性大于97%,并在系统进化树中与C4a亚型代表株处于同一分支,对89份样本VP1基因编码的氨基酸序列与安徽阜阳的FY23毒株进行了比较,发现89株病毒均在791位由S(丝氨酸)变为P(脯氨酸)。结论本研究所收集的2012-2013年广西桂林地区的89例EV71所致手足口病均为C4a亚型的EV71感染。
Objective To analyze the etiology of enterovirus 71 (EV71) in HFMD cases in Guilin, Guangxi during 2012-2013. Methods 737 samples of hand, foot and mouth disease were collected and detected by RT-PCR. EV71 positive samples were selected for virus isolation. VP1 gene was amplified by RT-PCR and sequenced. The VP1 gene was analyzed by evolutionary analysis. Results Among 737 cases, the positive rate of EV71 infection detected by fluorescence PCR was 169 cases (22.93%). The nucleotide and amino acid sequences of VP1 gene of 89 EV71 strains sequenced were 95.01% -100.00% and 98.47% 100.00%. The homology was more than 95% and the amino acid homology was more than 97% with the C4 subtype, and was in the same branch with C4a subtype in the phylogenetic tree. Compared with the FY23 strain in Fuyang, Anhui Province. The results showed that all the 89 strains changed from S (serine) to P (proline) at position 791. CONCLUSIONS: 89 cases of EV71-induced hand-foot-mouth disease in Guilin, Guangxi province during 2012-2013 were all EV71 infections of C4a subtype.