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目的:探讨乌灵菌粉(xylaria nigripes,XN)水提物对脑缺血再灌注模型小鼠海马形态和高分子量神经丝蛋白(neurofilament high molecular weight,NF-H)表达以及脑源性神经营养因子(brain derived neurotrophic factor,BDNF)和γ氨基丁酸(γ-aminobutyric acid,GABA)的影响。方法:将32只昆明小鼠随机分为对照组(sham)、模型组(MCAO)和乌灵菌粉水提物低、高剂量组,即XN(L)和XN(H)。sham组进行假手术(皮肤切开,分离颈动脉),模型组(MCAO)及乌灵菌粉组进行大脑中动脉闭塞(middle cerebral artery occlusion,MCAO),sham组和MCAO组术后即刻腹腔注射生理盐水,乌灵菌粉组则注射不同剂量的乌灵菌粉水提物(0.3 g/kg和0.6 g/kg),术后24 h,通过尼氏染色、免疫组化染色和酶联免疫吸附实验(enzyme-linked immunosorbent assay,Elisa)分别观察海马结构和NF-H的表达情况以及BDNF和GABA水平。结果:(1)模型组小鼠海马出现大量的空洞样改变,细胞排列不整齐,XN(H)组空洞样改变减少;(2)模型组NF-H染色的积分光密度显著低于对照组和XN(H)组,差异具有统计学意义(P<0.05);(3)Elisa检测显示,模型组海马BDNF和GABA水平显著低于对照组和XN(H)组,差异具有统计学意义(P<0.01),而且模型组与XN(L)组的GABA水平之间也存在显著性差异(P<0.05)。结论:乌灵菌粉水提物对MCAO模型小鼠海马结构具有保护作用,这一作用可能与其调节GABA和BDNF水平有关。
Objective: To investigate the effects of xylaria nigripes (XN) aqueous extract on the hippocampal formation and the expression of high-molecular-weight neurofilament protein (NF-H) and brain-derived neurotrophic factor (Brain derived neurotrophic factor, BDNF) and γ-aminobutyric acid (GABA). Methods: Thirty-two Kunming mice were randomly divided into sham, model group (MCAO) and low and high dose groups of water extract of Radix Astragali, namely XN (L) and XN (H). Sham group underwent sham operation (skin incision, carotid artery separation), model group (MCAO) and Wuling bacteria powder group, middle cerebral artery occlusion (MCAO), sham group and MCAO group Saline, Wuling powder group were injected different doses of Wuling powder water extract (0.3 g / kg and 0.6 g / kg), 24 h after surgery, by Nissl staining, immunohistochemical staining and enzyme-linked immunosorbent assay The expression of hippocampal formation and NF-H, as well as the levels of BDNF and GABA, were detected by enzyme-linked immunosorbent assay (Elisa). (2) The integral optical density of NF-H staining in model group was significantly lower than that in control group (P <0.05). (3) The Elisa test showed that the levels of BDNF and GABA in the hippocampus of the model group were significantly lower than those in the control group and the XN (H) group (P <0.05), and the difference was statistically significant P <0.01). There was also a significant difference (P <0.05) between GABA levels in model group and XN (L) group. Conclusion: The water extract of Ulmus pumila could protect the hippocampal formation of mice with MCAO, which may be related to the regulation of GABA and BDNF.