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目的：建立Ⅳ型胶原（ＣＩＶ）测定的放射免疫分析法。方法：从人胎盘组织提纯ＣＩＶ，免疫兔获得特异抗血清，制备１２５￣Ⅰ－ＣＩＶ标记抗原并建立ＣＩＶ放免分析法。用此法检测９０名正常人和１５０例各种肝病患者血清ＣＩＶ含量。结果：该法标准曲线工作范围２５～８００μｇ／Ｌ，灵敏度１０μｇ／Ｌ，批内和批间变异系数分别为４．７％和６．３％，平均回收率９５．０％。用该法测定正常人血清ＣＩＶ含量为９２．１±１１．０μｇ／Ｌ：慢活肝，肝硬化和原发性肝癌组血清ＣＩＶ水平显著高于正常组（Ｐ值＜０．０１）；急性肝炎组略有升高。慢活肝，肝硬化和原发性肝癌组高于急性肝炎和慢迁肝组（Ｐ值＜０．０１）。结论：建立的ＣＩＶ放射免疫分析法具有简便、灵敏、特异的优点，对肝纤维化及肝硬化的诊断具有临床实用价值。
Objective: To establish a radioimmunoassay for the determination of type Ⅳ collagen (CIV). Methods: CIV was purified from human placenta tissue, immunized rabbits were given specific antisera, 125I-CIV labeled antigen was prepared and CIV radioimmunoassay was established. This method was used to detect serum CIV levels in 90 normal subjects and 150 patients with various liver diseases. Results: The standard curve of this method has a working range of 25 ~ 800μg / L and a sensitivity of 10μg / L. The coefficient of variation (CV) was 4.7% and 6.3%, respectively. The average recovery was 95.0%. Using this method, the serum CIV level in normal people was 92.1 ± 11.0μg / L: serum CIV level in slow-living liver, cirrhosis and primary liver cancer was significantly higher than that in normal group (P <0.01); acute Hepatitis group increased slightly. Slow-active liver cirrhosis and primary liver cancer were higher than those in acute hepatitis and chronic liver transplantation (P <0.01). Conclusion: The established CIV radioimmunoassay has the advantages of simple, sensitive and specific, and has clinical value for the diagnosis of liver fibrosis and cirrhosis.