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目的研究小儿在轮状病毒急性感染期的免疫应答特点。方法采用ELISA方法检测血浆和粪便轮状病毒特异性抗体,流式细胞术进行淋巴细胞亚群分析,采用荧光定量RTPCR方法检测外周血单个核细胞转录因子、细胞因子mRNA的表达。结果腹泻患儿血浆中轮状病毒特异性IgMIgG抗体和大便中轮状病毒特异性IgA抗体及CD19细胞亚群比例,均较对照组有明显的升高。腹泻患儿外周血单个核细胞中IL12p40mRNA的表达升高。粪便和血浆中IgA抗体的滴度与患儿腹泻的严重程度有一定关系。结论急性轮状病毒感染小儿早期出现的免疫应答反应,以特异性抗体显著增加为主要特点,并且抗体产生的水平与疾病的严重程度相关。
Objective To study the characteristics of children’s immune response during the acute infection of rotavirus. Methods Serum and fecal rotavirus specific antibodies were detected by ELISA. Lymphocyte subsets were analyzed by flow cytometry. Fluorescent quantitative RTPCR was used to detect the expression of transcription factors and cytokines in peripheral blood mononuclear cells. Results The proportion of rotavirus specific IgM IgA antibody and stool rotavirus specific IgA antibody and CD19 cell subsets in children with diarrhea were significantly higher than those in control group. IL12p40mRNA expression in peripheral blood mononuclear cells of children with diarrhea increased. The titers of IgA antibodies in feces and plasma correlate with the severity of diarrhea in children. Conclusion The immune response in early stage of acute rotavirus infection in children is mainly characterized by the significant increase of specific antibodies, and the level of antibody production is related to the severity of the disease.